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ID PDW232 preliminary; circular DNA; SYN; 5510 BP.
XX
AC IG1062;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pDW232 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC [from none]
RA Becker D.M., Guarente L.;
RT "High-efficiency transformation of yeast by electroporation";
RL Meth. Enzymol. 194:182-187(1991).
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RN [2]
RC pON3 from pBR322 & pON1
RC pON5 from pON3 & lambda
RC pON163 from pON5 & ARS1
RC pDW222, pDW225 from HIS2 gene & URA gene
RC pDW227 from pGEM-3 & pON163
RC pDW229 from pGEM-3 & pON163
RC pDW232 from pGEM-3 & pDW227 & pDW229
RA Weilguny D., Praetorius M., Carr A.M., Egel R., Nielsen O.;
RT "New vectors in fission yeast: application for cloning the his2
RT gene";
RL Gene 99:47-54(1991).
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CC NM (pDW232)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Invitrogen)
CC HO (E.coli)(S.cerevisiae)(S.pombe)
CC CP ()
CC FN (expression)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pGEM-3 PvuII 2867bp 105..105
FT 2. pON163 ClaI-ClaI 1767bp 5651..7418, URA4 gene
FT Klenow:Klenow
FT -> pDW227 4634bp
FT 1. pGEM-3 NaeI 2867bp 2595..2595
FT 2. pON163 EcoRI-EcoRI 1198bp 4427..5625, ARS1
FT -> pDW229 4065bp
FT 1. pGEM-3 BglI-Asp700I 479bp 1294..1773, amp gene
FT 2. pDW229 Asp700I-HindIII 2300bp
FT \ 1773..2595..-..2595..2867..8, ARS1
FT 3. pDW227 HindIII-BglI 3053bp 8..105..-..105..1294,
FT \ URA4 gene
FT -> pDW232 5510bp"
FT promoter 0..0
FT /note="PRO bacteriophage Sp6"
FT misc_binding 0..0
FT /note="MCS HindIII-SphI-PstI-SalI-AccI-HincII
FT XbaI-BamHI-AvaI-SmaI-KpnI-SacI-EcoRI"
FT promoter complement(0..0)
FT /note="PRO bacteriophage T7"
FT CDS complement(0..0)
FT /note="ANT yeast URA4 gene"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="ANT E. coli neomycin phosphotransferase gene
FT (NPT), neomycin resistance gene (neo)"
FT rep_origin 0..0
FT /note="ORI yeast ARS1"
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SQ Sequence 5510 BP; 1607 A; 1215 C; 1123 G; 1565 T; 0 other;
gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
gcggatacag ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
cgcgcgtttc ggtgatgacg gtgaatgagg ccgttgagca ccgccgccgc aaggaatggt
gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc aattccaacc ttccaattca
ttaaatcgag tcctccatca gatgatttac aaggttttgc atagaatccc taagtaagag
aagttgttag tgatttcttc aagtttacta aaatttacat acaaatggat gatctcttct
ccattgtttc ctttcttctt gcaaacgcgt tttacaaaga gatgacatgc tattttctga
ttattttttt ctatagtttt ctattttgta cgtccttggg agtccgaaat gtaaaatcgg
ggtatggtta gtagcggcag cgttcattat ggtatataaa agataaaaag taggggcaag
cgagacagaa tgggatacaa gggcatcgtc tataattata gctaaaaatt gtattttaat
ttgtattttt tgtaatttta tttctcattg ttttacttaa aatgaatgcg aattagagaa
aacttcaacg aaatgtcaaa ataagctcag caaaatatac aattttaggg aaagcgatca
gcaacttatt ctccgttgta gattcttttt cttcataaat tccaacaatg taactgccaa
ttgatttttc tctagatgct aataaattga aactaatgaa tataataaca caggttttga
taaattgtaa actcttactc aaaaaaagaa tgttaaaact aatttaacct ttttataaag
gctcatggcg ttaaagagga tagtaaaaat tctacaattt tttaatttaa tgtcacaatt
gtcttgcgat taattgttgc cgggaagcta gagtaagagt tcgccagtta atagtttgcg
caacgttgtg ccattgctac aggcatcgtg gtgtcacgcc gtcgtttggt atggcttcat
tcagctccgg tcccaacgat caaggcgagt tacatgatcc ccatgttgtg caaaaaagcg
gttagctcct tcctgaccat ttccggggtg cggaacggcg ttaccagaaa ctcagaaggt
tcgtccaacc aaaccgactc tgacggcagt ttacgagaga gatgataggg tctgcttcag
taagccagat gctacacaat taggcttgta catattgtcg ttagaacgcg gctacaatta
atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa tacaagcttg
catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc cggtctccct
atagtgagtc gtattaattt cgataagcca gcgatagctt gtgatattga cgaaactttt
tgacatctaa tttattctgt tccaacacca atgtttataa ccaagtttta tcttgtttgt
ctacatggta ttttacattc atctacatac atctttcatt ggctttgtac atagttatca
ttacaagtct aaaaaaattc actcttttct tattcaatgt caatccaaga gaaaagattg
tggtaatgtt gtaggagcat gtttaataaa ttactatagc aaattacttt ttattcccaa
ggtgtttatc tataatagtt aatattttag tcgctacata aaattttacc aaagagtact
tgtatactaa ttctaaatgc cttctgacat aaaacgccta ggaaaacaaa cgcaaacaag
gcatcgactt tttcaataac caaccaaaaa aattttacat tagtcttttt ttaatgctga
gaaagtcttt gctgatatgc cttccaacca gcttctctat atctcttggc ttcgacaaca
ggattacgac cagctccata gactccacga ccaacaatga tgatatcgct accgcagttt
acaatcactt cttcaggagt acgatattgc tgtcccagcc cgtctccttt aacatccaag
ccgataccag gggacatagt tatgtagtcg ctttgaaggt taggaaatcg acgaccagct
ataaagccaa agcaaaaatc ggtatgcttc tcaaaccatt ctaaggtttt ctctgtgtag
gaaccagtag ccaaagagcc tttggaagac atttcagcca aaagcaagag accacgtccc
aaaggtaaac caacttcttt gaggccttgt ataataccct cgcctggcac tgtatggcaa
tttgtgatat gagcccaaga agcaattttg tacacaccag atgcatattg tagcttgacg
gtatttccaa tgtctgcgaa tttgcgatcc tcaaagataa gaaaacgatg ctttttacct
aaggccacca gtttttctac catatcctgg tcgaaatcct cgacaacgtc aatatgtgtc
ttgataacac agacataggg tccaatttta tctaccaatt ctaagatttc ggatttcttc
gtcaaatcga ccgcgactga caagttgctt tgcttttctt ccatcaaagc caacaattcc
ttggcaatgg gatttttcat cccctcagct ctagctgaat agctttgaaa tactctagca
tccataactt tgcttttaaa cctttaattt cgatccaagc aaaaaagagg ttcttggtag
gacaatacgg taagaaaaca cgacatgtgc agagatgccg acgaagcata gttaaactgg
gatggtaaaa tcaattaaga atttataaag acaaaattgt ataagtctct aaaacatctt
aattatacct cacagaacta tctaaaatat attcacaaag tgcaaacatt atcatgaaaa
agaaccattt taatttaaag caagggcatt aaggcttatt tacagaattt cttacttttg
taaagattat aaggctgatt atctttttca ccatgccaaa aattacacaa gatagaatgg
atgtttgaaa ttaaacgtga gtatacaaac aaatacacta ggtaaatcga aacatttttt
tctccattaa gtaacaaatt cctatttaga gaaagaatgc tgagtagatt aaataatcta
tacaaacttt tttaacacaa atgcatacat atagccagtg ggatttgtag ctaagctatc
gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
//