Return to this vector's summary.
ID PEBVHISA preliminary; circular DNA; SYN; 10317 BP.
XX
AC IG1063; K01383;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pEBVHisA - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pcDV1/S2 from pcDV1 & linker
RC pRSVCAT/H2 from pRSVcat & linker
RC [pBT from BT gene]
RC pRSV/BT from pRSVCAT/H2 & pBT
RC pRSVPA1 from pRSV/BT & pcDV1/S2
RC pRSVPA2 from pRSVPA1 & linker
RC p220.2 from p201
RC p220.2/B- from p220.2
RC pREP2 from pRSVPA2 & p220.2/B-
RC pREP3 from pREP2
RC p220.2/XS from p220.2/B-
RC pHS1PA4 from pRSVPA2
RC pMEP4 from p220.2/XS & pHS1PA4
RC pRSVCAT-B2 from pRSVcat & linker
RC pRSVCAT-B2K from pRSVCAT-B2 & linker
RC pREP4 from pRSVCAT-B2K & pMEP4
RC pREP5 from pREP4 & oligo
RA Groger R.K., Morrow D.M., Tykocinski M.L.;
RT "Directional antisense and sense cDNA cloning using Epstein-Barr
RT virus episomal expression vectors";
RL Gene 81:285-294(1989).
XX
CC NM (pEBVHisA)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Invitrogen)
CC HO (E.coli NM522)(E.coli INValphaF')(mammal)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (EBV)
CC BR (pEBVHisB)(pEBVHisC)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. EBV, EBNA-1
FT 2. SV40, polyA/tk gene
FT 3. RSV, LTR
FT 4. pBR322 4361bp
FT 5. E. coli 1142bp, hyg gene
FT -> pEBVHisA 10317bp"
FT misc_binding 1..1
FT /note="SIT XbaI"
FT polyA_signal 0..0
FT /note="PLA SV40 early gene"
FT misc_binding 0..0
FT /note="MCS"
FT misc_binding 1..1
FT /note="SIT enterokinase (EK)"
FT repeat_unit 0..0
FT /note="RPT histidine (6)"
FT misc_feature 0..0
FT /note="ATG"
FT promoter complement(0..0)
FT /note="PRO Rous sarcoma virus (RSV) long terminal
FT repeat (LTR)"
FT polyA_signal 0..0
FT /note="PLA SV40 thymidine kinase (TK) gene"
FT CDS complement(0..0)
FT /note="ANT E. coli hygromycin phosphotransferase gene;
FT hygromycin resistance gene (hyg)"
FT promoter complement(0..0)
FT /note="PRO SV40 thymidine kinase (TK) gene"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="GEN Epstein-Barr virus nuclear antigen gene
FT (EBNA-1)"
FT rep_origin 0..0
FT /note="ORI Epstein-Barr virus (EBV) oriP"
XX
SQ Sequence 10317 BP; 2528 A; 2591 C; 2933 G; 2265 T; 0 other;
tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct
tgccggcctc gatcgagcgg ccgctagcaa gcttcgaatt cccatatggt accagctgca
gatctcgagc tcggatcccc atcgatcctt atcgtcatcg tcgtacagat cccgacccat
ttgctgtcca ccagtcatgc tagccatacc atgatgatga tgatgatgag aaccccccat
ggaggtgcac accaatgtgg tgaatggtca aatggcgttt attgtatcga gctaggcact
taaatacaat tatctctgca atgcggaatt cagtggttcg tccaatccat gtcagacctg
tctgttgcct tcctaataag gcacgatcgt accaccttac ttccaccaat cggcatgcac
ggtgcttttt ctctccttgt aaggcatgtt gctaactcat cgttaccatg ttgcaagact
acaagtgtat tgcataagac tacatttccc cctccctatg caaaagcgaa actactatat
cctgagggga ctcctaaccg cgtacaaccg aagccccgct gggcgcctaa acacacccta
gtcccctcag atacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac
cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt
gctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta
tgcggcagca gagcagattg tactgagagt gcaccataca agctcaggat ctgcgatgat
aagctgtcaa acatgagaat tggtcgacca ctgggcgcca gaaatccgcg cggtggtttt
tgggggtcgg gggtgtttgg cagccacaga cgcccggtgt tcgtgtcgcg ccagtacatg
cggtccatgc ccaggccatc caaaaaccat gggtctgtct gctcagtcca gtcgtggacc
agaccccacg caacgcccaa aataataacc cccacgaacc ataaaccatt ccccatgggg
gaccccgtcc ctaacccacg gggccagtgg ctatggcagg gcctgccgcc ccgacgttgg
ctgcgagccc tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc
gggcgtattg gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc
gaaccccgcg tttatgaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt
gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc
catctcccct attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg
agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt
tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc
tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc
cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat
ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc
aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt
tcaggctttt tcatatctca ttgcccggga tctgcggcac gctgttgacg ctgttaagcg
ggtcgctgca gggtcgctcg gtgttcgagg ccacacgcgt caccttaata tgcgaagtgg
acctgggacc gcgccgcccc gactgcatct gcgtgttcga attcgccaat gacaagacgc
tgggcggggt ttgtgtcatc atagaactaa agacatgcaa atatatttct tccggggaca
ccgccagcaa acgcgagcaa cgggccacgg ggatgaagca gggcatggcg gccgacgcgc
tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcagcaaaag
gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
gaattctcat gtttgacagc ttatcatcgc gataagctga tcctcacagg ccgcacccag
cttttcttcc gttgccccag tagcatctct gtctggtgac cttgaagagg aagaggaggg
gtcccgagaa tccccatccc taccgtccag caaaaagggg gacgaggaat ttgaggcctg
gcttgaggct caggacgcaa atcttgagga tgttcagcgg gagttttccg ggctgcgagt
aattggtgat gaggacgagg atggttcgga ggatggggaa ttttcagacc tggatctgtc
tgacagcgac catgaagggg atgagggtgg gggggctgtt ggagggggca ggagtctgca
ctccctgtat tcactgagcg tcgtctaata aagatgtcta ttgatctctt ttagtgtgaa
tcatgtctga cgaggggcca ggtacaggac ctggaaatgg cctaggagag aagggagaca
catctggacc agaaggctcc ggcggcagtg gacctcaaag aagagggggt gataaccatg
gacgaggacg gggaagagga cgaggacgag gaggcggaag accaggagcc ccgggcggct
caggatcagg gccaagacat agagatggtg tccggagacc ccaaaaacgt ccaagttgca
ttggctgcaa agggacccac ggtggaacag gagcaggagc aggagcggga ggggcaggag
caggaggggc aggagcagga ggaggggcag gagcaggagg aggggcagga ggggcaggag
gggcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca ggaggggcag
gaggggcagg agcaggagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag
gaggaggggc aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag
gggcaggagg ggcaggagca ggaggagggg caggaggggc aggaggggca ggagcaggag
gaggggcagg agcaggaggg gcaggagggg caggaggggc aggagcagga ggggcaggag
caggaggagg ggcaggaggg gcaggagggg caggagcagg aggggcagga gcaggagggg
caggagcagg aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag
gggcaggagc aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag
caggaggagg ggcaggaggg gcaggagcag gaggggcagg aggggcagga gcaggagggg
caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag
gaggggcagg agcaggaggt ggaggccggg gtcgaggagg cagtggaggc cggggtcgag
gaggtagtgg aggccggggt cgaggaggta gtggaggccg ccggggtaga ggacgtgaaa
gagccagggg gggaagtcgt gaaagagcca gggggagagg tcgtggacgt ggagaaaaga
ggcccaggag tcccagtagt cagtcatcat catccgggtc tccaccgcgc aggccccctc
caggtagaag gccatttttc caccctgtag gggaagccga ttattttgaa taccaccaag
aaggtggccc agatggtgag cctgacgtgc ccccgggagc gatagagcag ggccccgcag
atgacccagg agaaggccca agcactggac cccggggtca gggtgatgga ggcaggcgca
aaaaaggagg gtggtttgga aagcatcgtg gtcaaggagg ttccaacccg aaatttgaga
acattgcaga aggtttaaga gctctcctgg ctaggagtca cgtagaaagg actaccgacg
aaggaacttg ggtcgccggt gtgttcgtat atggaggtag taagacctcc ctttacaacc
taaggcgagg aactgccctt gctattccac aatgtcgtct tacaccattg agtcgtctcc
cctttggaat ggcccctgga cccggcccac aacctggccc gctaagggag tccattgtct
gttatttcat ggtcttttta caaactcata tatttgctga ggttttgaag gatgcgatta
aggaccttgt tatgacaaag cccgctccta cctgcaatat cagggtgact gtgtgcagct
ttgacgatgg agtagatttg cctccctggt ttccacctat ggtggaaggg gctgccgcgg
agggtgatga cggagatgac ggagatgaag gaggtgatgg agatgagggt gaggaagggc
aggagtgatg taacttgtta ggagacgccc tcaatcgtat taaaagccgt gtattccccc
gcactaaaga ataaatcccc agtagacatc atgcgtgctg ttggtgtatt tctggccatc
tgtcttgtca ccattttcgt cctcccaaca tggggcaatt gggcataccc atgttgtcac
gtcactcagc tccgcgctca acaccttctc gcgttggaaa acattagcga catttacctg
gtgagcaatc agacatgcga cggctttagc ctggcctcct taaattcacc taagaatggg
agcaaccagc atgcaggaaa aggacaagca gcgaaaattc acgccccctt gggaggtggc
ggcatatgca aaggatagca ctcccactct actactgggt atcatatgct gactgtatat
gcatgaggat agcatatgct acccggatac agattaggat agcatatact acccagatat
agattaggat agcatatgct acccagatat agattaggat agcctatgct acccagatat
aaattaggat agcatatact acccagatat agattaggat agcatatgct acccagatat
agattaggat agcctatgct acccagatat agattaggat agcatatgct acccagatat
agattaggat agcatatgct atccagatat ttgggtagta tatgctaccc agatataaat
taggatagca tatactaccc taatctctat taggatagca tatgctaccc ggatacagat
taggatagca tatactaccc agatatagat taggatagca tatgctaccc agatatagat
taggatagcc tatgctaccc agatataaat taggatagca tatactaccc agatatagat
taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat
taggatagca tatgctatcc agatatttgg gtagtatatg ctacccatgg caacattagc
ccaccgtgct ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc ttggcgctca
ggcgcaagtg tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta tcttggcccg
cccacctact tatgcaggta ttccccgggg tgccattagt ggttttgtgg gcaagtggtt
tgaccgcagt ggttagcggg gttacaatca gccaagttat tacaccctta ttttacagtc
caaaaccgca gggcggcgtg tgggggctga cgcgtgcccc cactccacaa tttcaaaaaa
aagagtggcc acttgtcttt gtttatgggc cccattggcg tggagccccg tttaattttc
gggggtgtta gagacaacca gtggagtccg ctgctgtcgg cgtccactct ctttcccctt
gttacaaata gagtgtaaca acatggttca cctgtcttgg tccctgcctg ggacacatct
taataacccc agtatcatat tgcactagga ttatgtgttg cccatagcca taaattcgtg
tgagatggac atccagtctt tacggcttgt ccccacccca tggatttcta ttgttaaaga
tattcagaat gtttcattcc tacactagta tttattgccc aaggggtttg tgagggttat
attggtgtca tagcacaatg ccaccactga accccccgtc caaattttat tctgggggcg
tcacctgaaa ccttgttttc gagcacctca catacacctt actgttcaca actcagcagt
tattctatta gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg agagttcact
gcccgctcct tgatcttcag ccactgccct tgtgactaaa atggttcact accctcgtgg
aatcctgacc ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat atcgctgttc
cttaggaccc ttttactaac cctaattcga tagcatatgc ttcccgttgg gtaacatatg
ctattgaatt agggttagtc tggatagtat atactactac ccgggaagca tatgctaccc
gtttagggtt aacaaggggg ccttataaac actattgcta atgccctctt gagggtccgc
ttatcggtag ctacacaggc ccctctgatt gacgttggtg tagcctcccg tagtcttcct
gggcccctgg gaggtacatg tcccccagca ttggtgtaag agcttcagcc aagagttaca
cataaaggca atgttgtgtt gcagtccaca gactgcaaag tctgctccag gatgaaagcc
actcagtgtt ggcaaatgtg cacatccatt tataaggatg tcaactacag tcagagaacc
cctttgtgtt tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg gcaagttgta
ccaaccaact gaagggatta catgcactgc cccgaataca aaacaaaagc gctcctcgta
ccagcgaaga aggggcagag atgccgtagt caggtttagt tcgtccggcg gcggggc
//