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ID PEMBL8M preliminary; circular DNA; SYN; 3939 BP.
XX
AC X04996; ATCC37397;
XX
DT 07-JUN-1987 (Rel. 2, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phasmid vector pEMBL8- - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pEMBL8, pEMBL8+, pEMBL8- from pUC8 & pD4
RC plasmid from pEMBL8
RC lambda G7 from plasmid & lambda NM616
RC lambda G43 from lambda G7 & pUC9
RC pEMBL9, pEMBL9+, pEMBL9- from plasmid & lambda G43
RA Dente L., Cesareni G., Cortese R.;
RT "pEMBL: a new family of single stranded plasmids";
RL Nucleic Acids Res. 11:1645-1654(1983).
XX
RN [2]
RP 1-3939
RC pEMBL8-
RA Pfeiffer F.;
RT ;
RL Submitted (02-JUN-1987) on paper to the EMBL Data Library by:
RL Pfeiffer F., MPI Biochemie, Martinsried, Germany.
XX
RN [3]
RC pEMBL8, pEMBL8+, pEMBL8- from pD4 & pUC8
RC pEMBL9, pEMBL9+, pEMBL9- from pD4 & pUC9
RC pEMBL18+ from pEMBL8+ & M13mp18
RC pEMBL18- from pEMBL8- & M13mp18
RC pEMBL19+ from pEMBL8+ & M13mp19
RC pEMBL19- from pEMBL8- & M13mp19
RC pEMBL130+ from pEMBL8+ & M13tg130
RC pEMBL130- from pEMBL8- & M13tg130
RC pEMBL131+ from pEMBL8+ & M13tg131
RC pEMBL131- from pEMBL8- & M13tg131
RA Dente L., Cortese R.;
RT "pEMBL: a new family of single-stranded plasmids for sequencing DNA";
RL Meth. Enzymol. 155:111-119(1987).
XX
CC According to the cloning strategy, the phage f1 origin should
CC be flanked by regenerated EcoRI sites, but these are not found
CC experimentally.
CC The given sequence must be incorrect around this position.
CC The base n was introduced to destroy the EcoRI site (GAAnTTC).
CC ssDNA-producing plasmid with polylinker in lacZ'.
CC Medium is 1227 LB plus ampicillin.
CC NM (pEMBL8-)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phasmid)
CC SP (ATCC)
CC HO (E.coli 71/18)(E.coli)(E.coli JM series)
CC CP ()
CC FN (transcription)
CC SE ()
CC PA (pUC8)(M13mp8)
CC BR (pEMBL8+)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC8 NarI 2665bp 426..426, between amp & lac
FT 2. pD4 EcoRI-? 1260bp 4292..5552, f1 ori
FT -> pEMBL8- 3939bp"
FT misc_feature 1..3939
FT /note="pEMBL8- (minus) sequence"
FT misc_feature 1..423
FT /note="from pUC8 (bases 1..423)"
FT misc_binding 228..263
FT /note="MCS EcoRI-SmaI-BamHI-SalI-PstI-HindIII,
FT from M13mp8/pUC8-polylinker (bases 1..36)"
FT misc_feature 423..429
FT /note="destroyed EcoRI site (GAAnTTC) (bases 1..7)"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1 intergenic region"
FT misc_feature 431..1693
FT /note="from bacteriophage f1,
FT bases complement(5147..6407)"
FT mutation 745..748
FT /note="CTCT in pEMBL8-; CT in bacteriophage f1"
FT misc_feature 1694..1700
FT /note="destroyed EcoRI site (GAAnTTC) (bases 1..7)"
FT misc_feature 1700..3939
FT /note="from pUC8 (bases 426..2665)"
FT misc_feature 2205..2993
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp) (bases 1..789)"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_binding 0..0
FT /note="SIT ClaI"
FT misc_binding 0..0
FT /note="SIT EcoB"
FT misc_binding 0..0
FT /note="SIT NaeI"
XX
SQ Sequence 3939 BP; 1078 A; 939 C; 958 G; 962 T; 2 other;
gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgaa ttcccgggga
tccgtcgacc tgcagccaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
tggaanttcc aacatccaat aaatcataca ggcaaggcaa agaattagca aaattaagca
ataaagcctc agagcataaa gctaaatcgg ttgtaccaaa aacattatga ccctgtaata
cttttgcggg agaagccttt atttcaacgc aaggataaaa atttttagaa ccctcatata
ttttaaatgc aatgcctgag taatgtgtag gtaaagattc aaacgggtga gaaaggccgg
agacagtcaa atcaccatca atatgatatt caaccgttct agctgataaa ttcatgccgg
agagggtagc tatttttgag aggtctctac aaaggctatc aggtcattgc ctgagagtct
ggagcaaaca agagaatcga tgaacggtaa tcgtaaaact agcatgtcaa tcatatgtac
cccggttgat aatcagaaaa gccccaaaaa caggaagatt gtataagcaa atatttaaat
tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
cgccgcgctt aatgcgccgc tacagggcgc gtactatggt tgctttgacg agcacgtata
acgtgctttc ctcgttagaa tcagagcggg agctaaacag gaggccgatt aaagggattt
tagacaggaa cggtacgcca gaatcctgag aagtgttttt ataatcagtg aggccaccga
gtaaaagagt ctgtccatca cgcaaattaa ccgttgtcgc aatacttctt tgattagtaa
taacatcact tgcctgagta gaagaactca aactatcggc cttgctggta atatccagaa
caatattacc gccagccatt gcaacaggaa aaacgctcat ggaaatacct acattttgac
gctcaatcgt ctggaanttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
ccgagcgcag cgagtcagtg agcgaggaag cggaagagc
//