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ID PFL26 preliminary; circular DNA; SYN; 5558 BP.
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AC X70477; ATCC77199;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Saccharomyces/E.coli plasmid vector pFL26 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-5558
RC pFL26
RA Ozier-Kalogeropoulos O.;
RT ;
RL Submitted (01-JUN-1993) by:
RL Ozier-Kalogeropoulos O., CGM, CNRS, 91190 Gif sur Yvette, France.
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RN [2]
RP 1-5558
RC plasmid from pUC19 & linkers
RC pFL26 from plasmid & YEp13, LEU2 gene
RC pFL34 from plasmid & pBR322-URA3, URA3 gene
RC pFL35 from plasmid & YRp7, TRP1 gene
RC pFL36 from pFL26 & CEN6 & ARS
RC pFL38 from pFL34 & CEN6 & ARS
RC pFL39 from pFL35 & CEN6 & ARS
RC pFL46S from pFL26 & yeast 2 micron & linker
RC pFL44S from pFL34 & yeast 2 micron & linker
RC pFL45S from pFL35 & yeast 2 micron & linker
RC pFL46L from pFL26 & pFL1 & linker
RC pFL44L from pFL34 & pFL1 & linker
RC pFL45L from pFL35 & pFL1 & linker
RC pFL59- from pFL39 & pGEM-3Zf-
RC pFL59+ from pFL44S & pGEM-3Zf+
RC pFL64- from pFL39 & pGEM-3Zf-
RC pFL64+ from pFL44S & pGEM-3Zf+
RA Bonneaud N., Ozier-Kalogeropoulos O., Li G.Y., Labouesse M.,
RA Minvielle-Sebastia L., Lacroute F.;
RT "A family of low and high copy replicative, integrative and
RT single-stranded S. cerevisiae/E. coli shuttle vectors";
RL Yeast 7:609-615(1991).
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CC pFL26 was constructed from pUC19 plasmid where two alu I sites
CC were modified.The site 629 was replaced by a BglII linker and the
CC site 747 by a ClaI site. The yeast selectable marker has been
CC cloned in the BglII site.
CC
CC The order of the major features in this plasmid is:EcoRI/MCS/HindIII -
CC LEU2 - pMB1 ori - ampR.
CC YI-type shuttle integration vector permitting visual detection of
CC recombinants based on alpha complementation.
CC Restriction digests of the clone give the following sizes (kb):
CC BamHI--5.6; BglII--2.8 (doublet); ClaI--3.6, 1.9. (ATCC staff)
CC Medium is 1227 LB plus ampicillin.
CC NCBI gi: 397127
CC NM (pFL26)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli HB101)(S.cerevisiae)(E.coli)(Saccharomyces)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pUC19)(yeast 2-micron plasmid)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC19 AluI 2686bp 631..631
FT BglII linker 6bp agatct
FT AluI 2693bp 747..747
FT ClaI linker 6bp atcgat
FT -> plasmid 2703bp
FT 1. plasmid BglII 2703bp 630..630
FT 2. YEp13 BglII-BglII 2855bp, yeast LEU2 gene
FT -> pFL26 5558bp"
FT misc_binding 0..0
FT /note="MCS EcoRI-BanII-SacI-Asp718-KpnI-AvaI-SmaI-
FT XmaI-BamHI-XbaI-
FT AccI-HincII-SalI-BspM1-PstI-SphI-HindIII"
FT misc_binding 0..0
FT /note="SIT unique SacI-SmaI-XmaI-BamHI-XbaI-PstI-
FT SphI-HindIII"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT promoter 0..0
FT /note="PRO E. coli lac gene"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="ANT yeast LEU2 gene"
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SQ Sequence 5558 BP; 1586 A; 1218 C; 1251 G; 1503 T; 0 other;
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
gctttccagt cgggaaacct gtcgtgccag cagatctatt acattatggg tggtatgttg
gaataaaaat caactatcat ctactaacta gtatttacgt tactagtata ttatcatata
cggtgttaga agatgacgca aatgatgaga aatagtcatc taaattagtg gaagctgaaa
cgcaaggatt gataatgtaa taggatcaat gaatattaac atataaaatg atgataataa
tatttataga attgtgtaga attgcagatt cccttttatg gattcctaaa tcctcgagga
gaacttctag tatatctaca tacctaatat tattgcctta ttaaaaatgg aatcccaaca
attacatcaa aatccacatt ctcttcaaaa tcaattgtcc tgtacttcct tgttcatgtg
tgttcaaaaa cgttatattt ataggataat tatactctat ttctcaacaa gtaattggtt
gtttggccga gcggtctaag gcgcctgatt caagaaatat cttgaccgca gttaactgtg
ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca ttattttttt
cctcaacata acgagaacac acaggggcgc tatcgcacag aatcaaattc gatgactgga
aattttttgt taatttcaga ggtcgcctga cgcatatacc tttttcaact gaaaaattgg
gagaaaaagg aaaggtgaga gccgcggaac cggcttttca tatagaatag agaagcgttc
atgactaaat gcttgcatca caatacttga agttgacaat attatttaag gacctattgt
tttttccaat aggtggttag caatcgtctt actttctaac ttttcttacc ttttacattt
cagcaatata tatatatata tttcaaggat ataccattct aatgtctgcc cctaagaaga
tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac agccgaagcc attaaggttc
ttaaagctat ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat catttaattg
gtggtgctgc tatcgatgct acaggtgtcc cacttccaga tgaggcgctg gaagcctcca
agaaggttga tgccgttttg ttaggtgctg tgggtggtcc taaatggggt accggtagtg
ttagacctga acaaggttta ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa
gaccatgtaa ctttgcatcc gactctcttt tagacttatc tccaatcaag ccacaatttg
ctaaaggtac tgacttcgtt gttgtcagag aattagtggg aggtatttac tttggtaaga
gaaaggaaga cgatggtgat ggtgtcgctt gggatagtga acaatacacc gttccagaag
tgcaaagaat cacaagaatg gccgctttca tggccctaca acatgagcca ccattgccta
tttggtcctt ggataaagct aatgttttgg cctcttcaag attatggaga aaaactgtgg
aggaaaccat caagaacgaa ttccctacat tgaaggttca acatcaattg attgattctg
ccgccatgat cctagttaag aacccaaccc acctaaatgg tattataatc accagcaaca
tgtttggtga tatcatctcc gatgaagcct ccgttatccc aggttccttg ggtttgttgc
catctgcgtc cttggcctct ttgccagaca agaacaccgc atttggtttg tacgaaccat
gccacggttc tgctccagat ttgccaaaga ataaggtcaa ccctatcgcc actatcttgt
ctgctgcaat gatgttgaaa ttgtcattga acttgcctga agaaggtaag gccattgaag
atgcagttaa aaaggttttg gatgcaggta tcagaactgg tgatttaggt ggttccaaca
gtaccacgga agtcggtgat gctgtcgccg aagaagttaa gaaaatcctt gcttaaaaag
attctctttt tttatgatat ttgtacataa actttataaa tgaaattcat aatagaaacg
acacgaaatt acaaaatgga atatgttcat agggtagacg aaactatata cgcaatctac
atacatttat caagaaggag aaaaaggagg atgtaaagga atacaggtaa gcaaattgat
actaatggct caacgtgata aggaaaaaga attgcacttt aacattaata ttgacaagga
ggagggcacc acacaaaaag ttaggtgtaa cagaaaatca tgaaactatg attcctaatt
tatatattgg aggattttct ctaaaaaaaa aaaaatacaa caaataaaaa acactcaatg
acctgaccat ttgatggagt ttaagtcaat accttcttga accatttccc ataatggtga
aagttccctc aagaatttta ctctgtcaga aacggcctta acgacgtagt cgacctcctc
ttcagtacta aatctaccaa taccaaatct gatggaagaa tgggctaatg catcatcctt
acccagcgca tgtaaaacat aagaaggttc tagggaagca gatgtacagg ctgaacccga
ggataatgcg atatccctta gtgccatcaa taaagattct ccttccacgt aggcgaaaga
aacgttaaca caccctggat aacgatgatc tggagatccg ttcaacgtgg tatgttcagc
ggataataga cctttgacta atttatcgga tagtcttttg atgtgagctt ggtcgttgtc
aaattctttc ttcatcaatc tcgcagcttc accaaatccc gctaccaatg ggggggccaa
agtaccagat ctgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
agcggtatca gcatcgatgc tcactcaaag gtcggtaata cggttatcca cagaatcagg
ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
aacctataaa aataggcgta tcacgaggcc ctttcgtc
//