ID   PGEM1      preliminary; circular DNA; SYN; 2865 BP.
XX
AC   X65300; IG0045;
XX
DT   17-JUN-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pGEM-1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pGEM-1, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
RN   [2]
RP   1-2865
RC   pGEM-1
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
RN   [3]
RC   pGEM-1 from pUC13
RA   Lewis K.;
RT   ;
RL   Unpublished (1986).
RL   Lewis K., Promega.
XX
CC   old Promega vector.
CC   NM (pGEM-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(transcription)
CC   SE (color blue/white)
CC   PA (pUC13)
CC   BR (pGEM-2)(pGEM-3)(pGEM-4)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC13 2680bp, ori/amp/lacZ
FT                   2. T7, promoter
FT                   3. Sp6, promoter
FT                   -> pGEM-1 2865bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   misc_feature    7..57
FT                   /note="MCS HindIII-PstI-SalI-BamHI-SmaI-EcoRI"
FT   misc_feature    66..66
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   promoter        61..83
FT                   /note="PRO bacteriophage T7"
FT   misc_feature    94..152
FT                   /note="GEN E. coli lac operon"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        join(2848..2865,1..6)
FT                   /note="PRO bacteriophage Sp6"
XX
SQ   Sequence 2865 BP; 710 A; 737 C; 729 G; 689 T; 0 other;
     gaatacaagc ttgggctgca ggtcgactct agaggatccc cgggcgagct cgaattccgg
     tctccctata gtgagtcgta ttaatttcga taagccagct gcattaatga atcggccaac
     gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc
     gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc
     ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
     agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
     gccggtgatg ccggccacga tgcgtccggc gtagaggatc tggctagcga tgaccctgct
     gattggttcg ctgaccattt ccggggtgcg gaacggcgtt accagaaact cagaaggttc
     gtccaaccaa accgactctg acggcagttt acgagagaga tgatagggtc tgcttcagta
     agccagatgc tacacaatta ggcttgtaca tattgtcgtt agaacgcggc tacaattaat
     acataacctt atgtatcata cacatacgat ttaggtgaca ctata
//