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ID PGEX4T3 preliminary; circular DNA; SYN; 4968 BP.
XX
AC IG1076; M21676; M97937;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pGEX-4T3 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC plasmid from pSj1 & pIC19H
RC pSj10 from plasmid & ptac or ptac12deltaEco
RC p4.5 from pSj5 & pMC9
RC pSj10Bam7Stop7 from pSj10
RC pGEX-1 from pBR322 & pSj10Bam7Stop7 & p4.5 & oligo
RC pGEX-2T from pGEX-1 & oligo, thrombin protease site
RC pGEX-3X from pGEX-1 & oligo, factor Xa site
RA Smith D.B., Johnson K.S.;
RT "Single-step purification of polypeptides expressed in Escherichia
RT coli as fusions with glutathione S-transferase";
RL Gene 67:31-40(1988).
XX
CC thrombin or factor Xa protease sites to cleave protein from fusion.
CC pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs.
CC NM (pGEX-4T3)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Pharmacia)
CC HO (E.coli)
CC CP ()
CC FN (expression)(cloning directional)
CC SE (affinity)(color yellow)
CC PA (pGEX-2T)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pGEX-4
FT 2. oligo, thrombin site
FT -> pGEX-4T-3 4968bp"
FT misc_binding 0..0
FT /note="MCS unique EcoRI-SmaI-SalI-XhoI-NotI"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 0..0
FT /note="SIT unique PstI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="REP E. coli lacI repressor gene"
FT misc_binding 0..0
FT /note="SIT unique MluI"
FT misc_binding 0..0
FT /note="SIT unique BstEII"
FT misc_binding 0..0
FT /note="SIT unique ApaI"
FT misc_binding 0..0
FT /note="SIT unique BssHII"
FT misc_binding 0..0
FT /note="SIT unique EcoRV"
FT misc_binding 0..0
FT /note="SIT unique NarI"
FT promoter 0..0
FT /note="PRO E. coli tac (trp and lac)"
FT CDS 0..0
FT /note="GEN Schistosoma japonicum
FT glutathione S-transferase (GST)"
FT misc_binding 0..0
FT /note="SIT thrombin"
XX
SQ Sequence 4968 BP; 1225 A; 1202 C; 1291 G; 1250 T; 0 other;
acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
atcctccaaa atcggatctg gttccgcgtg gatccccgaa ttcccgggtc gactcgagcg
gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg tgatacgcct
atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca taaattccga
caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca
actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc
gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt
ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct
cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc
catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt
cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg
tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga
acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga
gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga
taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct
gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca
aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
aatttcacac aggaaacagc tatgaccatg attacggatt cactggccgt cgttttacaa
cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
cacggttacg atgcgcccat ctacaccaac gtaacctatc ccattacggt caatccgccg
tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
tggctacagg aaggccagac gcgaattatt tttgatggcg ttggaatt
//