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ID PGEX5X3 preliminary; circular DNA; SYN; 4974 BP.
XX
AC IG1079; M21676; M97937;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pGEX-5X3 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC plasmid from pSj1 & pIC19H
RC pSj10 from plasmid & ptac or ptac12deltaEco
RC p4.5 from pSj5 & pMC9
RC pSj10Bam7Stop7 from pSj10
RC pGEX-1 from pBR322 & pSj10Bam7Stop7 & p4.5 & oligo
RC pGEX-2T from pGEX-1 & oligo, thrombin protease site
RC pGEX-3X from pGEX-1 & oligo, factor Xa site
RA Smith D.B., Johnson K.S.;
RT "Single-step purification of polypeptides expressed in Escherichia
RT coli as fusions with glutathione S-transferase";
RL Gene 67:31-40(1988).
XX
CC thrombin or factor Xa protease sites to cleave protein from fusion.
CC pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs.
CC NM (pGEX-5X3)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Pharmacia)
CC HO (E.coli)
CC CP ()
CC FN (expression)(cloning directional)
CC SE (affinity)(color yellow)
CC PA (pGEX-3X)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pGEX-5 4968bp
FT 2. oligo, factor Xa site
FT -> pGEX-5X-3 4974bp"
FT misc_binding 0..0
FT /note="MCS unique BamHI-EcoRI-SmaI-SalI-XhoI-NotI"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 0..0
FT /note="SIT unique PstI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="REP E. coli lacI repressor gene"
FT misc_binding 0..0
FT /note="SIT unique MluI"
FT misc_binding 0..0
FT /note="SIT unique BstEII"
FT misc_binding 0..0
FT /note="SIT unique ApaI"
FT misc_binding 0..0
FT /note="SIT unique BssHII"
FT misc_binding 0..0
FT /note="SIT unique EcoRV"
FT misc_binding 0..0
FT /note="SIT unique NarI"
FT promoter 0..0
FT /note="PRO E. coli tac (trp and lac)"
FT CDS 0..0
FT /note="GEN Schistosoma japonicum
FT glutathione S-transferase (GST)"
FT misc_binding 0..0
FT /note="SIT factor Xa"
XX
SQ Sequence 4974 BP; 1229 A; 1201 C; 1294 G; 1250 T; 0 other;
agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
atcctccaaa atcggatctg atcgaaggtc gtgggatccc caggaattcc cgggtcgact
cgagcggccg catcgtgact gactgacgat ctgcctcgcg cgtttcggtg atgacggtga
aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg gcctcgtgat
acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataaa
ttccgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
gataacaatt tcacacagga aacagctatg accatgatta cggattcact ggccgtcgtt
ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat tacggtcaat
ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttgg aatt
//