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ID PIB8 preliminary; circular DNA; SYN; 5406 BP.
XX
AC U00003;
XX
DT 01-DEC-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli cosmid vector pIB8 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-5406
RC pIB8 from HomerI
RA Lakey N., Ghazizadeh H., Gryan G., Jaehn L., Mintz L.,
RA Richterich P., Robison K., Church G.M.;
RT "Complete sequence of cosmid vector pIB";
RL Unpublished (1993).
XX
RN [2]
RP 1-5406
RC pIB8
RA Robison K.;
RT ;
RL Submitted (05-MAY-1993) by:
RL Robison K., .
XX
CC pIB8 was derived from pHomerI by insertion of a BamHI site at a
CC completely filled-in EcoRI site. [2]
CC pHomerI was derived from pAT153 [3] by insertion of the cos region
CC of Charon 4 into a BstI site. [4]
CC NM (pIB8)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (cosmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pHomerI from pAT153 and Charon 4)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. HomerI EcoRI
FT fill in
FT BamHI linker 6bp ggatcc
FT -> pIB8 5406bp"
FT misc_binding join(5406,1..5)
FT /note="SIT BamHI; cloning site"
FT misc_binding 7..12
FT /note="SIT EcoRI; for liberating insert"
FT misc_feature 10..5401
FT /note="from pHomerI; pIB8 was constructed from
FT pHomer by filling in the unique EcoRI site and
FT ligating-in a BamHI linker"
FT CDS 218..1078
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 2038..2043
FT /note="SIT HaeII; marking deletion of
FT copy-number controlling region of pBR322"
FT misc_feature complement(join(2415..3306,5021..5336))
FT /note="remnant of tetA gene, destroyed during pHomerI
FT construction; also 2 frameshifts."
FT misc_binding 3010..3015
FT /note="SIT SalI; used to generate left arm"
FT misc_binding 4609..4620
FT /note="SIT phage lambda cohesive site; cos gene
FT lacking a coding region."
FT misc_binding 5370..5375
FT /note="SIT HindIII; used to generate left arm"
FT misc_binding 5399..5405
FT /note="SIT EcoRI; for liberating insert"
XX
SQ Sequence 5406 BP; 1354 A; 1350 C; 1441 G; 1261 T; 0 other;
gatccggaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
tttgtgatgc tcgtcagggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gctgacttcc gcgtttccag
actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt
gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag
gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg
ccaggaccca acgctgcccg agatgccgcg tgcggctgct ggagatggcg gacgcgatgg
atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc
caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt
ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc
tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat
cagcggtcca atgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc
ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcggcat cccgatgccg
ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc
aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa
cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc
gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc
cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg
gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
agtcccccgg ccacgggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcttg gcagtcacag
catgcgcata tccatgcttc gaccatgcgc tcacaaagta ggtgaatgcg caatgtagta
cccacatcgt catcgctttc cactgctctc gcgaataaag atggaaaatc aatctcatgg
taatagtcca tgaaaatcct tgtattcata aatcctccag gtagctatat gcaaattgaa
acaaaagaga tggtgatctt tctaagagat gatggaatct cccttcagta tcccgatggt
caatgcgctg gatatgggat agatgggaat atgctgattt ttatgggaca gagttgcgaa
ctgttcccaa ctaaaatcat tttgcacgat cagcgcacta cgaactttac ccacaaatag
tcaggtaatg aatcctgata taaagacagg ttgataaatc agtcttctac gcgcatcgca
cgcgcacacc gtagaaagtc tttcagttgt gagcctgggc aaaccgttaa ctttcggcgg
ctttgctgtg cgacaggctc acgtctaaaa ggaaataaat catgggtcat aaaattatca
cgttgtccgg cgcggcgacg gatgttctgt atgcgctgtt tttccgtggc gcgttgctgt
ctggtgatct gccttctaaa tctggcacag ccgaattgcg cgagcttggt tttgctgaaa
ccagacacac agcaactgaa taccagaaag aaaatcactt tacctttctg acatcagaag
ggcagaaatt tgccgttgaa cacctggtca atacgcgttt tggtgagcag caatattgcg
cttcgatgac gcttggcgtt gagattgata cctctgctgc acaaaaggca atcgacgagc
tggaccagcg cattcgtgac accgtctcct tcgaacttat tcgcaatgga gtgtcattca
tcaaggacgc cgctatcgca aatggtgcta tccacgcagc ggcaatcgaa acacctcagc
cggtgaccaa tatctacaac atcagccttg gtatccagcg tgatgagcca gcgcagaaca
aggtaaccgt cagtgccgat aagttcaaag ttaaacctgg tgttgatacc aacattgaaa
cgttgatcga aaacgcgctg aaaaacgctg ctgaatgtgc ggcgctggat gtcacaaagc
aaatggcagc agacaagaaa gcgatggatg aactggcttc ctatgtccgc acggccatca
tgatggaatg tttccccggt ggtgttatct ggcagcagtg ccgtcgatag tatgcaattg
ataattatta tcatttgcgg gtcctttccg gcgatccgcc ttgttacggg gcggcgacct
cgcgggtttt cgctatttat gaaaattttc cggtttaagg cgtttccgtt cttcttcgtc
ataacttaat gtttttattt aaaataccct ctgaaaagaa aggaaacgac aggtgctgaa
agcgagcttt ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga atgaacaatg
gaagtcaaca aaaagcagct ggctgacatt ttcggtgcga gtatccgtac cattcagaac
tggcaggaac agggaatgcc cgttctgcga ggcggtggca agggtaatga ggtgctttat
gactctgccg ccgtcataaa atggtatgcc gaaagggatg ctgaaattga gaacgaaaag
ctgcgccggg aggttgaaga actgcggcag gccagcgagg cagatccaca ggacgggtgt
ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg
gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc
atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg
caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt
gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca
atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga
attccg
//