Return to this vector's summary.
ID PNO1523 preliminary; circular DNA; SYN; 4799 BP.
XX
AC IG0092; ATCC37095; J01686; V00357; J01688; V00354; M12490; M12432;
AC M12489;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pNO1523 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pNO1517 from lambda & pBR322
RC pNO1523 from pNO1517
RA Dean D.;
RT "A plasmid cloning vector for the direct selection of strains carrying
RT recombinant plasmids";
RL Gene 15:99-102(1981).
XX
RN [2]
RC pNO2005 from lambda fus3 & pVH51
RA Post L.E., Nomura M.;
RT "DNA sequences from the str operon of E. coli";
RL J. Biol. Chem. 255:4660-4666(1980).
XX
RN [3]
RC [lambda spc2delta9 from lambda spc2]
RC from lambda spc2delta9
RC from phage Ch3S45i lambda
RA Post L.E., Arfsten A.E., Davis G.R., Nomura M.;
RT "DNA sequence of the promoter region for the alpha ribosomal protein
RT operon in E. coli";
RL J. Biol. Chem. 255:4653-4659(1980).
XX
RN [4]
RC lambda fus3 from lambda
RA Post L.E., Arfsten A.E., Reusser F., Nomura M.;
RT "DNA sequences of promoter regions for the str and spc ribosomal
RT protein operons in E.coli";
RL Cell 15:215-229(1978).
XX
CC ATCC size is 5200 bp.
CC old Pharmacia vector.
CC A vector for positive selection of clones with inserts based on the
CC dominance of the plasmid coded rpsL+ gene. [1]
CC Medium is 1227 LB plus ampicillin.
CC NM (pNO1523)
CC NM (pNO1523)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli MC1009)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pNO1517)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pNO1517 remove BstEII-SalI, S12
FT DNA polymerase I
FT -> pNO1523 4799bp [no SalI, no BstEII]"
FT promoter complement(518..891)
FT /note="PRO E. coli S12 gene"
FT promoter complement(894..937)
FT /note="PRO E. coli rpsL+ reporter gene"
FT misc_binding 0..0
FT /note="SIT unique SmaI"
FT misc_binding 0..0
FT /note="SIT unique HpaI"
FT misc_binding 0..0
FT /note="SIT unique SstII/SacII"
FT rep_origin 3043..3043
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(3801..4661)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT promoter 0..0
FT /note="PRO E. coli streptomycin resistance gene"
FT CDS 0..0
FT /note="ANT E. coli streptomycin resistance gene (str)"
FT CDS 0..0
FT /note="GEN E. coli rpsL+ reporter gene"
XX
SQ Sequence 4799 BP; 1130 A; 1293 C; 1225 G; 1151 T; 0 other;
agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
ctgtggatcc ggcagaattt tacgctgacc aatgacgcga cgacgtggca tggaaatact
ccgttgttaa ttcaggattg tccaaaactc tacgagttta gtttgacatt taagttaaaa
cgtttggcct tacttaacgg agaaccatta agccttagga cgcttcacgc catacttgga
acgagcctgc ttacggtctt taacgccgga gcagtcaagc gcaccacgta cggtgtggta
acgaacaccc gggaggtctt taacacgacc gccacggatc aggatcacgg agtgctcctg
caggttgtga ccttcaccac cgatgtagga agtcacttcg aaaccgttag tcagacgaac
acggcatact ttacgcagcg cggagttcgg ttttttagga gtggtagtat atacacgagt
acatacgcca cgtttttgcg ggcatgcttc cagcgcaggc acgttgcttt tcgcaacttt
gcgagcacgt ggttgcgtac cagctggtta actgttgcca ttaaatagct cctggtttta
gcttttgctt cgtaaacacg taataaaacg tcctcacaca atatgaggac gccgaatttt
agggcgatgc cgaaaaggtg tcaagaaata tacaacgatc ccgccatcat caccaggcca
tctggctggg gtgcttaacc gtaagtctga cgaaatcagt atagtcaatg agaatgatgt
cgttcgaaat ttgaccagtc aaaccgcggg caataaggtc ttcgttcagg gcatagacct
taatgggggc attacgcaga ctttcaaggt agcggttact cagctccttc cggtgggcgc
ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa
cttcgatcac tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga
acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc
gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg
attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac
caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat
ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac
ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg
aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt
cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt
ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac
gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag
ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt
gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatt cccccttaca
cggaggcatc aagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga
agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac
atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc
ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//