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ID POM3 preliminary; circular DNA; SYN; 2713 BP.
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AC L08939; VB0060; ATCC77222;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli plasmid vector pOM3 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pUC830 from pUC8 & M13tg131
RC pUC931 from pUC9 & M13tg131
RC pOM1 from pUC8 & M13tg131
RC pOM2 from pUC9 & M13tg131
RC pOM3 from pUC8 & M13tg131
RC pOM4 from pUC9 & M13tg131
RC pOM8 from pUC8 & M13tg131
RC pOM9 from pUC9 & M13tg131
RA Oberbaumer I.;
RT "New pUC-derived expression vectors for rapid construction of cDNA
RT libraries";
RL Gene 49:81-91(1986).
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RN [2]
RC from pOM series
RA ;
RT ;
RL Focus 14:72-72(1992).
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RN [3]
RP 1-2713
RC pOM3
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
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CC These data and their annotation were supplied to GenBank by Will
CC Gilbert under the auspices of the GenBank Currator Program.
CC
CC Assembled by F. Pfeiffer, MPI Biochemie, Martinsried
CC Expression vector useful for construction of cDNA libraries and
CC containing priming sites useful for sequencing. Permits visual
CC detection of recombinants by beta-galactosidase alpha peptide
CC complementation. [1]
CC pOM3 (ATCC 77222) and pOM4 (ATCC 77223) differ in the orientation of
CC the polylinker. [1]
CC Contains an additional ATG in the inserted ClaI linker in a different
CC reading frame which might also function as start codon for expression.
CC [1]
CC Restriction digests of the clone give the following sizes (kb):
CC PstI--2.7; HindIII--2.7; EcoRI--2.7. (ATCC staff)
CC Medium is 1227 LB plus ampicillin.
CC NM (pOM3)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli HB101)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (M13tg131)(pUC9)(pUC931)(bGal131)
CC BR (pOM1)(pOM2)(pOM4)(pOM5)(pOM6)(pOM7)(pOM8)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC9 HindIII 2665bp 234..234
FT mung bean nuclease
FT ClaI linker 8bp natcgatn
FT remove PstI-EcoRI 18bp 246..264, MCS/2650bp
FT 2. M13tg131 PstI-EcoRI 59bp 6242..6301, MCS
FT -> pOM3 2713bp"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT promoter 0..0
FT /note="PRO E. coli lac gene"
FT CDS 0..0
FT /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT CDS 981..1769
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_feature 1..233
FT /note="from pUC9"
FT misc_feature 235..240
FT /note="SIT ClaI linker"
FT misc_feature 248..316
FT /note="MCS ClaI-PstI-SalI-BamHI-HindIII-XbaI-KpnI-
FT SphI-EcoRV-SstI-SmaI-EcoRI; M13tg131-Polylinker"
FT misc_feature 306..2713
FT /note="from pUC9"
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SQ Sequence 2713 BP; 664 A; 693 C; 682 G; 674 T; 0 other;
gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccacatcgat
ggcttggctg caggtcgacg gatcccaagc ttcttctaga ggtaccgcat gcgatatcga
gctctcccgg gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
aggaagcgga aga
//