ID   EMBL12     preliminary; circular DNA; SYN; 43200 BP.
XX
AC   IG1002; ATCC37485;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda EMBL12 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   EMBL12 from EMBL3 & pUC12
RA   Natt E., Scherer G.;
RT   "EMBL12, a new lambda replacement vector with sites for SalI,
RT   XbaI, BamHI, SstI, and EcoRI";
RL   Nucleic Acids Res. 14:7128-7128(1986).
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CC   Phage with inserts have the Spi- phenotype.
CC   Medium is 1592 SM buffer.
CC   NM (EMBL12)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP (Boehringer)(ATCC)
CC   HO (E.coli LE392)(E.coli Q358)(E.coli Q359)(E.coli NM538)
CC   HO (E.coli NM539)
CC   CP ()
CC   FN (cloning 8000 to 23000 bp)(in vitro packaging)
CC   SE ()
CC   PA (EMBL3)
CC   BR ()
CC   OF ()
CC   OR (E.coli Y1090)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. EMBL3 remove SalI-BamHI-EcoRI 22bp
FT                   \ 20045..20067, 29215bp
FT                   2. pUC12 EcoRI-SalI 30bp 231..261, MCS
FT                   3. stuffer 13300bp
FT                   4. pUC12 EcoRI-SalI 30bp 231..261, MCS
FT                   -> EMBL12 43200bp"
FT   misc_binding    19900..20000
FT                   /note="MCS SalI-XbaI-BamHI-SacI/SstI-EcoRI"
FT   misc_feature    20000..33300
FT                   /note="stuffer fragment"
FT   misc_binding    33300..33400
FT                   /note="MCS EcoRI-SacI/SstI-BamHI-XbaI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda Spi+ reporter gene"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//