ID   FBH16      preliminary; DNA; SYN; 9100 BP.
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AC   ATCC37001;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector fd fBH 16 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   fKN16 from fd-tet
RC   [fBH16 from fKN16 & linker]
RA   Nelson F.K., Friedman S.M., Smith G.P.;
RT   "Filamentous phage DNA cloning vectors: a noninfective mutant with
RT   a nonpolar deletion in gene III";
RL   Virology 108:338-350(1981).
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CC   Provided as lyophilized viral DNA. When reconstituted with 50 ul
CC   sterile distilled water, the DNA concentration is about 10x10(11)
CC   ssDNA molecules/ml in 1% SDS, 10 mM Tris pH8, 10 mM HCl, 1 mM EDTA.
CC   Same as fKN16 (ATCC 37002) with a BamHI site added. (ATCC staff)
CC   fd bacteriophage vector. (ATCC staff)
CC   Medium is 1592 SM buffer.
CC   NM (fBH 16)
CC   CM (no)
CC   NA (filamentous ss-DNA)
CC   TP (linear)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (bacteriophage fd)(E.coli C600)(E.coli K802)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (bacteriophage fd)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. fd-tet, Tn10 tet
FT                   -> fKN16
FT                   1. fKN16 9100bp, Tn10 tet
FT                   BamHI linker 6bp ggatcc
FT                   -> fBH16 9100bp"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage fd"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//