ID   LAMBLUMIDM preliminary; circular DNA; SYN; 40100 BP.
XX
AC   IG1005;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phage vector lambda BLUEMID- - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   lambda BLUEMID+, lambda BLUEMID- from lambda & lacZ gene
RA   ;
RT   ;
RL   Unpublished (1992).
XX
CC   NM (lambda BLUEMID-)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (CLONTECH)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning cDNA)
CC   SE ()
CC   PA (lambda)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda, cos
FT                   2. T3, promoter
FT                   3. E. coli, lacI gene
FT                   4. pBR322, ori/amp gene
FT                   5. M13, ori
FT                   6. E. coli, lacZ gene
FT                   7. T3, promoter
FT                   -> lambda BLUEMID- 40100bp"
FT   misc_binding    0..0
FT                   /note="SIT lambda cos"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRV"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage M13 ori+"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT lambda cos"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//