ID   LAMFIXII   preliminary; circular DNA; SYN; 43000 BP.
XX
AC   IG2042; X04125; X16973;
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DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda FIX II - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   lambda FIX II from lambda
RA   Elgin E.;
RT   ;
RL   Strategies 4:6-7(1991).
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CC   NM (lambda FIX II)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (Stratagene)
CC   HO (E.coli XL-1-Blue MRA)(E.coli XL-1-Blue MRA P2)
CC   CP ()
CC   FN (cloning 9000-23000 bp)(cloning genomic)(expression prokaryote)
CC   SE ()
CC   PA (lambda)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda, cos
FT                   2. T3, promoter
FT                   3. T7, promoter
FT                   -> lambda FIX II 43000bp"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cosL)"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda A"
FT   promoter        20000..20000
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    0..0
FT                   /note="MCS XbaI-SacI-NotI-SacI-SalI-XhoI-EcoRI-XbaI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda J"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda red+"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda gam+"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda N"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda cro"
FT   promoter        complement(34000..34000)
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS XbaI-EcoRI-XhoI-SalI-SacI-NotI-SacI-XbaI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda O"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda P"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda Q"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda R"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cosL)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//