ID   LAMGEM12   preliminary; circular DNA; SYN; 43000 BP.
XX
AC   IG1010;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phage vector lambda GEM12 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   from Charon 4A
RA   Liu X.Y., Maeda S., Collier K., Pestka S.;
RT   "Rapid identification of genomic interferon genes by sequencing on
RT   phage lambda DNA templates";
RL   Gene Anal. Tech. 2:83-88(1985).
XX
RN   [2]
RC   from EMBL3
RC   [M13MC18, M13MC19 from M13 & lambda, cos; hyphage vectors]
RC   [lambda GEM2, lambda GEM4, lambda GEM11, lambda GEM12 from lambda]
RA   Zabarovsky E.R., Allikmets R.L.;
RT   "An improved technique for the efficient construction of gene
RT   libraries by partial filling-in of cohesive ends";
RL   Gene 42:119-123(1986).
XX
CC   left arm is 20000 bp. right arm is 9000 bp.
CC   NM (lambda GEM12)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (Promega)
CC   HO (E.coli LE392)(E.coli KW251)(E.coli NM538)(E.coli NM539)
CC   CP ()
CC   FN (cloning 9000-23000 bp)(chromosome walking)
CC   SE ()
CC   PA (lambda GEM11)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda GEM12 43000bp"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   misc_binding    0..0
FT                   /note="SIT KpnI"
FT   misc_binding    0..0
FT                   /note="SIT KpnI"
FT   misc_binding    0..0
FT                   /note="SIT SmaI"
FT   misc_binding    0..0
FT                   /note="SIT double SfiI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS SacI-NotI-BamHI-EcoRI-XhoI-XbaI"
FT   misc_feature    0..0
FT                   /note="lambda 2001 stuffer fragment (14000 bp)"
FT   CDS             0..0
FT                   /note="GEN E. coli red+"
FT   CDS             0..0
FT                   /note="GEN E. coli gam+"
FT   misc_binding    0..0
FT                   /note="SIT SmaI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="MCS XbaI-XhoI-EcoRI-BamHI-NotI-SacI"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage Sp6"
FT   misc_binding    0..0
FT                   /note="SIT double SfiI"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT SmaI"
FT   misc_binding    0..0
FT                   /note="SIT SstI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//