ID   LAMGEM4    preliminary; circular DNA; SYN; 46200 BP.
XX
AC   IG1012;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phage vector lambda GEM4 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   from lambda gt10
RC   from lambda gt11
RA   Jendrisak J., Young R.A., Engel J.D.;
RT   "Cloning cDNA into lambda gt10 and lambda gt11";
RL   Meth. Enzymol. 152:359-371(1987).
XX
CC   pGEM-1 and lambda have unique SpeI site.
CC   NM (lambda GEM4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (Promega)
CC   HO (E.coli C600)(E.coli C600hflA)(E.coli LE392)
CC   CP ()
CC   FN (cloning cDNA)(cloning up to 7100 bp)(cloning directional)
CC   SE ()
CC   PA (lambda)(pGEM-1)
CC   BR ()
CC   OF ()
CC   OR (E.coli Y1090)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda 40000bp
FT                   2. pGEM-1 2865bp
FT                   -> lambda GEM4 46200bp"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda A"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda J"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda b527"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda imm434"
FT   misc_binding    32700..32700
FT                   /note="SIT unique SpeI"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   misc_binding    0..0
FT                   /note="MCS XbaI-SacI-EcoRI"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    33300..33300
FT                   /note="SIT unique SpeI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda cI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cos)"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//