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ID LAMGT11 preliminary; circular DNA; SYN; 43700 BP.
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AC IG0304; X16973; X04125; ATCC37194;
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DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phage vector lambda gt11 - incomplete, SacI-KpnI + lac/junct.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC [lambda gt7-lac5 from lambda gt7 & lac gene]
RC phage from lambda gt7-lac5 & lambda 540
RC lambda gt11 or lambda plac5 lacZ from phage & lambda gt4
RA Young R.A., Davis R.W.;
RT "Efficient isolation of genes by using antibody probes";
RL Proc. Natl. Acad. Sci. U.S.A. 80:1194-1198(1983).
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RN [2]
RC from lambda gt11 or lambda plac5 lacZ
RA Young R.A., Davis R.W.;
RT "Yeast RNA polymerase II genes: isolation with antibody probes";
RL Science 222:778-782(1983).
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RN [3]
RC from lambda gt11 or lambda plac5 lacZ
RA Mierendorf R.C., Percy C., Young R.A.;
RT "Gene isolation by screening lambda gt11 libraries with
RT antibodies";
RL Meth. Enzymol. 152:458-469(1987).
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RN [4]
RP 1-1989
RC lambda gt11 or lambda plac5 lacZ, SacI-KpnI
RA Slatko B.E.;
RT ;
RL Submitted (20-OCT-1989) by:
RL Slatko B.E., New England Biolabs, Inc., 32 Tozer Road,
RL Beverly MA 01915, USA.
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RN [5]
RP 1-1989
RC from lambda gt11 or lambda plac5 lacZ, SacI-KpnI
RC from lambda plac5 or lambda cI857 lacZ
RA Moran L.S., Maina C.V., Poole C.B., Slatko B.E.;
RT "Nucleotide sequence of the phage lambda-gt11 SacI-KpnI lacZ region";
RL Gene 93:163-164(1990).
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RN [6]
RP 1-210
RC from lambda gt11 or lambda plac5 lacZ, lac gene/lambda phage junction
RA Chirala S.S.;
RT "The nucleotide sequence of the lac operon and phage junction in
RT lambda gt11";
RL Nucleic Acids Res. 14:5935-5935(1986).
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CC Left EcoRI arm = 19600 bp. Right EcoRI arm = 24100 bp.
CC The cI857 repressor is temperature sensitive and so is inactive
CC at 42 degrees but active at 32 degrees, allowing selection against
CC lysogeny.
CC Medium is 1592 SM buffer.
CC NCBI gi: 58246 & 58114
CC NM (lambda gt11)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)(linear)
CC ST ()
CC TY (phage)
CC SP (Boehringer)(BRL)(CLONTECH)(Invitrogen)(NEB)(Pharmacia)(Promega)
CC SP (Sigma)(Stratagene)(USB)(Worthington)(ATCC)
CC HO (E.coli Y1088)(E.coli Y1089)(E.coli Y1089r-)
CC HO (E.coli Y1090)(E.coli Y1090r-)(E.coli C600)
CC CP ()
CC FN (cloning 7000-7200 bp)(cloning cDNA)(expression)
CC FN (antibody screening)(in vitro packaging)
CC SE (color blue/white)
CC PA (lambda)
CC BR ()
CC OF ()
CC OR (E.coli Y1090)
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. lambda
FT -> lambda gt4 [lambda cI857 S100 nin5]
FT 1. lambda
FT -> lambda 540 [lambda deltaB imm21 nin5]
FT 1. lambda
FT -> lambda gt7 [lambda b522 nin5]
FT 1. lambda gt7
FT 2. E. coli lac gene
FT -> lambda gt7-lac5 [lambda lac5 b522 nin5]
FT 1. lambda gt7-lac5 left end-HindIII, left arm
FT 2. lambda 540 HindIII-right end, right arm
FT -> phage
FT 1. lambda gt4, right arm
FT recombination
FT 2. phage, left arm
FT -> lambda gt11 43700bp
FT \ [lambda lac5 cI857 nin5 Sam100; unique EcoRI]"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cohesive end site
FT (cosL)"
FT misc_binding 0..0
FT /note="SIT BamHI"
FT CDS 0..0
FT /note="GEN bacteriophage lambda A"
FT CDS 0..0
FT /note="GEN bacteriophage lambda J"
FT misc_binding 0..0
FT /note="SIT PvuI"
FT misc_feature 1..6
FT /note="SIT SacI"
FT misc_feature 1069..1074
FT /note="SIT EcoRI"
FT misc_feature 1984..1989
FT /note="SIT KpnI"
FT misc_binding 0..0
FT /note="SIT KpnI"
FT misc_binding 0..0
FT /note="SIT KpnI"
FT CDS complement(0..0)
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT misc_feature 1..109
FT /note="bacteriophage lambda gt11 lom region"
FT promoter 80..83
FT /note="PRO E. coli lac; pot. -35 region"
FT promoter 100..105
FT /note="PRO E. coli lac; pot. -10 region"
FT misc_feature 110..110
FT /note="junction phage/lac operon"
FT RBS 124..129
FT /note="RBS; potential"
FT misc_feature complement(111..>210)
FT /note="lac operon 3' end"
FT CDS 135..>210
FT /note="GEN bacteriophage lambda open reading frame;
FT NCBI gi: 58115"
FT misc_binding 19600..19600
FT /note="SIT unique EcoRI; in C-terminus of lacZ"
FT promoter complement(0..0)
FT /note="PRO E. coli lacUV5 gene"
FT misc_binding 0..0
FT /note="SIT SacI"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT misc_binding 24820..24820
FT /note="SIT unique XbaI"
FT misc_binding 0..0
FT /note="SIT BamHI"
FT rep_origin 0..0
FT /note="ORI bacteriophage lambda"
FT misc_binding 31500..31500
FT /note="SIT unique XhoI"
FT misc_binding 0..0
FT /note="SIT BamHI"
FT misc_binding 0..0
FT /note="SIT PvuI"
FT CDS 0..0
FT /note="GEN bacteriophage lambda shndIII2-3"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT CDS 0..0
FT /note="GEN bacteriophage lambda cI857"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT CDS 0..0
FT /note="GEN bacteriophage lambda nin5"
FT CDS 0..0
FT /note="GEN bacteriophage lambda S100 amber (Sam100)"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cohesive end site
FT (cosL)"
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SQ Sequence 2310 BP; 519 A; 647 C; 580 G; 453 T; 111 other;
gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct
ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag
agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca
gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg
ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc
gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg
attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg
gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc
tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca
cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta
tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat
gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg
caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc
cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac
gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac
cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa
accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc
catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg
agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg
ccatgtctgc ccgtatttcg cgtaaggaaa tccattgtac tgccggacca ccgactgtga
gccactccgg ccatggcgta cgcactgacc tgcttactga tttgtaaaac cggtccggcc
atcacgctca cataacgtcc acgcaggctc tcatagtgaa acgtatcctc cccggtcatc
actgtgctgc tctttttcga cgcggcgaac cccagggaag ccatcacccc cacactgtcc
gtcagctcat aacggtactt cacgttaatc cctttcagat gactcacacc ggtatccccg
cccgacaacg acggcaatgt acccggtttc acttgaaaat agcccaccgt aaacgtacca
tgtccacctt ccgcacgggc cggagtgact gtcaccgcaa gtgcggcaaa gacagcaacg
gcaatacaca cattacgcat cgttcacctc tcactgtttt ataataaaac gcccgttccc
ggacgaacct ctgtaacaca ctcagaccac gctgatgccc agcgcctgtt tcttaatcac
cataacctgc acatcgctgg caaacgtata cggcggaata tctgccgaat gccgtgtgga
cgtaagcgtg aacgtcagga tcacgtttcc ccgacccgct ggcatgtcaa caatacggga
gaacacctgt accgcctcgt tcgccgcgcc atcataaatc accgcaccgt tcatcagtac
tttcagataa cacatcgaat acgttgtcct gccgctgaca gtacgcttac ttccgcgaaa
cgtcagcgga agcaccacta tctggcgatc aaaaggatgg tcatcggtca cggtgacagt
acgggtaccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
acgttatgtg agcgtgatgg ccggaccggt tttacaaatc agtaagcagg tcagtgcgta
cgccatggcc ggagtggctc acagtcggtg gtccggcagt acaatggatt tccttacgcg
aaatacgggc agacatggcc tgcccggtta ttattatttt tgacaccaga ccaactggta
atggtagcga ccggcgctca gctggaattc
//