ID   LAMGT22A   preliminary; circular DNA; SYN; 42730 BP.
XX
AC   IG1015;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda gt22A - incomplete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   lambda gt22 from lambda gt18 & chi sequence & linker
RA   Han J.H., Rutter W.J.;
RT   "Lambda gt22, an improved lambda vector for the directional
RT   cloning of full-length cDNA";
RL   Nucleic Acids Res. 15:6304-6304(1987).
XX
RN   [2]
RC   lambda gt18 from lambda gt11 & oligo
RC   lambda gt19 from lambda gt11 & oligo
RA   Han J.H., Stratowa C., Rutter W.J.;
RT   "Isolation of full-length putative rat lysophospholipase cDNA
RT   using improved methods for mRNA isolation and cDNA cloning";
RL   Biochemistry 26:1617-1625(1987).
XX
CC   Left EcoRI arm = 19600 bp. Right EcoRI arm = 24100 bp.
CC   The cI857 repressor is temperature sensitive and so is inactive
CC   at 42 degrees but active at 32 degrees, allowing selection against
CC   lysogeny.
CC   NM (lambda gt22A)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)(linear)
CC   ST ()
CC   TY (phage)
CC   SP (BRL)
CC   HO (E.coli Y1088)(E.coli Y1089)(E.coli Y1089r-)
CC   HO (E.coli Y1090)(E.coli Y1090r-)
CC   CP ()
CC   FN (fusion)(cloning cDNA up to 8200 bp)(expression)
CC   FN (antibody screening)(cloning cDNA random/oriented)
CC   SE (color blue/white)
CC   PA (lambda gt11)
CC   BR (lambda gt22)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda gt11 EcoRI 43700bp 19600..19600
FT                   2. oligo SalI-EcoRI
FT                   -> lambda gt18 43700bp
FT                   \ [unique SalI][lambda lacZ cI857 Sam100]
FT                   1. lambda gt18 XbaI 43700bp 24820..24820
FT                   fill in
FT                   SacI, lacUV5 gene
FT                   fill in
FT                   SalI, lacUV5 gene
FT                   oligo NotI-XbaI-SacI-SalI
FT                   2. chi sequence
FT                   -> lambda gt22 42730bp [lambda lacZ cI857 Sam100]"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cos)"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda A"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda J"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_feature    0..0
FT                   /note="lambda gt11 reverse primer"
FT   misc_binding    <19595..>19630
FT                   /note="MCS unique NotI-XmaI-XbaI-SpeI-SalI-EcoRI"
FT   misc_feature    0..0
FT                   /note="lambda gt11 forward primer"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lacUV5 gene"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   misc_binding    30500..30500
FT                   /note="SIT unique XhoI"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda cI857"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda S100 amber (Sam100)"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cohesive end site
FT                   (cos)"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//