ID   LAMGTWEST5 preliminary; circular DNA; SYN; 37800 BP.
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AC   ATCC37068;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda gtWES.T5-622 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   lambda gtWES.T5-622 from lambda gtWES.lambdaB' & phage T5
RA   Davison J., Brunel F., Merchez M.;
RT   "A new host-vector system allowing selection for foreign DNA
RT   inserts in bacteriophage lambda gtWES";
RL   Gene 8:69-80(1979).
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RN   [2]
RC   lambda gtWES.T5-6 from lambda gtWES.lambdaB & T5
RC   lambda gtWES.T5-622 from lambda gtWES.T5-6
RA   Davison J., Brunel F., Merchez M.;
RT   "Construction of lambdagtWES.T5-622";
RL   DNA - recombination interactions and repair 0:55-64(1980).
RL   Zadravil S., Sponar J., eds., Pergamum, Oxford.
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RN   [3]
RC   lambda gt.lambdaC from lambda
RC   lambda gt.lambdaBC from lambda
RC   lambda gt.lambdaDm from lambda
RC   lambda gt-O from lambda gt.lambdaBC
RA   Thomas M., Cameron J.R., Davis R.W.;
RT   "Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA";
RL   Proc. Natl. Acad. Sci. U.S.A. 71:4579-4583(1974).
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RN   [4]
RC   lambda gtWES.lambdaB from lambda gtWES.lambdaC & lambda gt.lambdaB
RC   [lambda gtWES.lambdaB' from lambda gtWES.lambdaC & lambda gt.lambdaB]
RC   NOTE: WES = Wam403 Eam1100 Sam100
RA   Tiemeier D., Enquist L., Leder P.;
RT   "An improved derivative of a bacteriophage lambda EK2 vector
RT   useful in the cloning of recombinant DNA molecules:
RT   lambda gtWES.lambdaB";
RL   Nature 263:526-527(1976).
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RN   [5]
RC   [lambda gt.lambdaB from lambda]
RC   lambda gtWES.lambdaC from lambda gt.lambdaC
RA   Enquist L., Tiemeier D., Leder P., Weisberg R., Sternberg N.;
RT   "Safer derivatives of bacteriophage lambdagt-lambdaC for use in
RT   cloning of recombinant DNA molecules";
RL   Nature 259:596-598(1976).
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CC   A cloning vector permitting positive selection for inserts. (ATCC
CC   staff)
CC   The lower limit for inserts is about 2.38 Kb.
CC   Medium is 1592 SM buffer.
CC   NM (lambda gtWES.T5-622)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phage)
CC   SP (ATCC)
CC   HO (bacteria-free lysate)(E.coli)(E.coli LE392 [for propagation])
CC   HO (E.coli MM21 [for recombinant])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (lambda gtWES.T5-6 from lambda gtWES.lambdaB from lambda gtWES)(T5)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   duplicate a200 in left arm, to make longer
FT                   substitute b189 for b2, remove EcoRI 1/EcoRI 2
FT                   substitute bio69 for int, remove EcoRI 3
FT                   -> lambda a200 b189 bio69 cI857 nin5, cI857/nin5
FT                   1. lambda a200 b189 bio69 cI857 nin5, cI857/nin5
FT                   EcoRI, EcoRI 4/lambda 39169..39169
FT                   \ lambda EcoRI = 21227 26105 31748 39169 44973
FT                   mutagenesis
FT                   EcoRI, EcoRI 5/lambda 44973..44973
FT                   mutagenesis
FT                   recombination, between beta and N genes
FT                   2. lambda N-, left arm/B fragment/C fragment
FT                   -> lambda gt.lambdaBC
FT                   1. lambda gt.lambdaBC
FT                   remove EcoRI-EcoRI 5400bp, C fragment
FT                   \ lambda 5643bp 26105..31748
FT                   -> lambda gt.lambdaB [left arm/B fragment/right arm]
FT                   1. lambda gt.lambdaBC EcoRI-EcoRI
FT                   remove EcoRI-EcoRI 4690bp, B fragment
FT                   \ lambda 4878bp 21227..26105
FT                   -> lambda gt.lambdaC [left arm/C fragment/right arm]
FT                   1. lambda gt.lambdaC
FT                   mutation Wam403
FT                   mutation Eam100
FT                   mutation Sam100
FT                   -> lambda gtWES.lambdaC
FT                   1. lambda gt.lambdaB EcoRI, lambda B fragment
FT                   2. lambda gtWES.lambdaC EcoRI, left arm/right arm
FT                   -> lambda gtWES.lambdaB [B frag in middle]
FT                   1. T5 mutated
FT                   -> T5ris1ris3ris4 [three EcoRI sites at each end]
FT                   1. lambda gtWES.lambdaB EcoRI
FT                   2. T5 EcoRI(1)-EcoRI(3), 5' end
FT                   -> lambda gtWES.T5-6
FT                   1. lambda gtWES.T5-6 remove EcoRI-EcoRI, lambdaB
FT                   -> lambda gtWES.T5-622 37800bp [2 T5 fragments]"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage lambda"
FT   CDS             0..0
FT                   /note="GEN bacteriophage T5 A2 gene;
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="GEN bacteriophage T5 A3 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//