ID   LAMMGU1    preliminary; circular DNA; SYN; 44900 BP.
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AC   S46756; IG1360;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phage vector lambda MGU1 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [lambda 2690 from lambda]
RC   lambda MGU2 from lambda 2690 & modified gam gene
RC   pKG1 from pKSS & modified gam gene
RC   pUM113 from BlueScribe M13+
RC   pKG2 from pKG1 & pUM113
RC   pLP1 from pRH536 & oligo
RC   pMGU from pKG2 & pLP1
RC   lambda MGU1 from pMGU & lambda 2690
RC   lambda MGU2 from lambda MGU1
RC   pCRE1 from pRH147 & pBR322
RA   Maruyama I.N., Brenner S.;
RT   "A selective lambda phage cloning vector with automatic excision
RT   of the insert in a plasmid";
RL   Gene 120:135-141(1992).
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RN   [2]
RC   lambda loxP2 from lambda & phage P1
RC   lambda loxP2 rev from lambda loxP2
RC   lambda loxP spi- from lambda loxP2
RC   lambda loxP rev spi- from lambda loxP2 rev
RC   pRH31 from pRH30
RC   pRH31-39 from pRH31 & linker
RC   pRH31-56 from pRH31 & linker
RC   pRH3156-24 from pRH31-56 & linker
RC   pRH40 from pRH31 & linker
RC   pRH41 from pRH40 & linker
RC   pRH42 from pRH41 & pRH3156-24
RC   pKB12 from Tn5 kan gene
RC   pRH43 from pRH42 & pKB12, Tn5 kan gene
RC   pRH44, pRH45 from pRH43
RC   pRH100 from pRH31-39 & phage P1
RC   [pRH147 from phage P1]
RC   [pRH536 from phage P1]
RA   Abremski K., Hoess R., Sternberg N.;
RT   "Studies on the properties of P1 site-specific recombination:
RT   evidence for topologically unlinked products following recombination";
RL   Cell 32:1301-1311(1983).
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CC   NM (lambda MGU1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (linear)
CC   ST ()
CC   TY (phage)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning cDNA)
CC   SE ()
CC   PA (lambda 2690)(pMGU)
CC   BR ()
CC   OF (lambda MGU2)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> lambda 2690 40700bp
FT                   1. pMGU SalI 4185bp 964..964
FT                   2. lambda 2690 SalI, lambda 32746 or 33245/40700bp
FT                   -> lambda MGU1 44900bp [losP-pMGU-loxP]"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cos"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRV"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_feature    0..0
FT                   /note="from pMGU"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T3"
FT   CDS             complement(0..0)
FT                   /note="REP E. coli lacI repressor gene"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      complement(0..0)
FT                   /note="ORI bacteriophage M13 ori-"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT bacteriophage lambda cos"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//