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ID LAMSK17 preliminary; circular DNA; SYN; 51000 BP.
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AC ATCC86969;
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DT 01-FEB-1995 (Rel. 11, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phage lambdaSK17 - incomplete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC placZ from pBR322 & Charon 4A, lacZ gene
RC phage EL3/4 from EMBL3 & EMBL4 & placZ
RC lambda SK3 from EMBL3 & phage EL3/4
RC phage SK-P from EMBL4 & linker
RC lambda SK5 from lambda SK3 & phage SK-P
RC lambda SK1, lambda SK2, lambda SK6 from pHC79 & M13mp18
RC lambda SK18, lambda SK18' from pHC79 & M13mp18
RC lambda SK2A from lambda SK18
RC lambda SK2deltaSR from lambda SK2
RC lambda SK9 from EMBL3 & lambda SK2deltaSR
RC lambda SK10 from lambda SK9 & oligo
RC lambda SK11 from lambda gt11 & oligo
RC lambda SK12 from lambda SK2A & phage EL3/4
RC lambda SK15 from lambda SK9 & pUC4K
RC lambda SK16 from lambda SK15 & oligo
RC lambda SK17 from lambda SK15 & oligo
RC lambda SK20 from lambda SK10 & oligo
RC lambda SK21 from lambda SK9 & pGEM-11Zf+
RC lambda SK22 from lambda SK9 & oligo
RC pSR9 from lambda SK2
RC lambda SK23 from lambda SK10 & oligo & pSR9
RC lambda SK24 from lambda SK22 & lambda SK23
RC M13mc18 from M13mp18 & pHC79
RC M13mc19 from M13mp19 & pHC79
RA Zabarovsky E.R., Winberg G.;
RT "Improved methods and new vectors for creating the clone libraries
RT of genes";
RL Sov. Sci. Rev. D. Physiochem. Biol. 11:1-65(1992).
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RN [2]
RC lambda SK4 from EMBL3 & oligo
RC lambda SK6 from lambda 2001 & oligo
RC lambda SK10 from lambda SK9 & oligo
RC lambda SK20 from lambda SK10 & oligo
RC lambda SK21 from lambda SK9 & lambda GEM11
RC lambda SK22 from lambda SK9 & oligo
RC lambda SR9-B from lambda SK18 & oligo
RC lambda SK23 from lambda SK10 & EMBL3 & lambda SR9-B
RC lambda SK24 from lambda SK22 & EMBL3 & lambda SR9-B
RC lambda SK25 from lambda SK22 & oligo
RC lambda SK27 from lambda SK22
RC lambda SK40 from lambda SK10 & oligo
RC lambda SK41 from lambda SK10 & oligo
RC lambda SK16 from lambda SK15 & oligo
RC lambda SK17 from lambda SK15 & oligo
RC lambda SK28 from lambda SK17
RA Zabarovsky E.R., Klein G., Winberg G.;
RT "Lambda SK diphasmids: phage lambda vectors for genomic, jumping,
RT linking and cDNA libraries";
RL Gene 127:1-14(1993).
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CC Restriction digests of the clone give the following sizes (kb):
CC BamHI--25.0, 13.5 (doublet); EcoRI--25.0, 13.5 (doublet); SalI--21.0,
CC 8.8, 8.2, 5.0, 4.4, 3.6. (ATCC staff)
CC Expression vector permitting positive selection (Spi) of
CC recombinants and control of expression using ochre (supB, supC and/or
CC supG, supV) and amber (supE, supF) suppressors. Useful for conversion
CC to phagemid.
CC Permits expression in three reading frames, construction using
CC partial filling-in, and oriented cloning. The sizes of the major
CC components are (kb): arms--20.3, 12.7; stuffer fragment--13.7;
CC phagemid segments--3.9 (longer arm), 0.7. The polylinker sites given
CC are those associated with the longer arm. The sites associated with
CC the other arm are: SalI BamHI EcoRI XhoI NotI NaeI SfiI AvrII NheI
CC NcoI XbaI. The restriction sites to be used for conversion to phagemid
CC are: SalI, ClaI.
CC Cloning capacity: 0.2 - 15.4 kb. The order of the major features in
CC this plasmid is: arm - ClaI - BspMII - SalI - pMB1 ori - ampR -
CC M13 ori - SacI/MCS/ SalI --> - stuffer - SalI/MCS/XbaI/lacZ' -
CC ClaI - SalI - arm. [2]
CC Deposited by: Zabarovsky E.R., Winberg G.R., Klein G.R.
CC NM (lambdaSK17)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)(linear)
CC ST ()
CC TY (phage)
CC SP (ATCC)
CC HO (E.coli)
CC CP ()
CC FN (cloning)(expression)(DNA library)
CC SE (Spi+)
CC PA ()
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. lambda 48502bp, ori
FT 2. M13, ori
FT 3. pBR322, ori
FT -> lambdaSK17 51000bp"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI E. coli phage lambda"
FT rep_origin 0..0
FT /note="ORI E. coli phage M13"
FT CDS 0..0
FT /note="ANT E. coli red+ gene"
FT CDS 0..0
FT /note="ANT E. coli gam+ gene"
FT misc_binding 0..0
FT /note="MCS SacI-KpnI-SmaI-NcoI-NheI-AvrII-SfiI-NaeI-
FT NotI-XhoI-EcoRI-BamHI-SalI/SalI-BamHI-EcoRI-XhoI-
FT NotI-NaeI-AvrII-NheI-NcoI-XbaI-PvuI-PstI-SphI-HindIII"
FT promoter 0..0
FT /note="PRO E. coli lac"
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SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
n
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