Return to this vector's summary.
ID LAMZAPII preliminary; circular DNA; SYN; 1 BP.
XX
AC IG2040; X04125; X16973;
XX
DT 03-FEB-1994 (Rel. 8, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phage vector lambda ZAP II - incomplete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pJF3 from pUC19 & T7 oligo
RC pBS or BlueScribe from pJF3 & T3 oligo
RC pBS+, pBS- from pBS or BlueScribe & pEMBL8
RC pBluescript KS(+) from pBS+ & linker
RC pBluescript KS(-) from pBS- & linker
RC pBST-B from pBS & pEMBL8
RC pBSITO#12 from pBST-B & pEMBL8
RC pPreB from pBSITO#12 & pBluescript SK(-)
RC lambda cI857 Sam7, lambda cI857 Sam100 nin5 from lambda
RC lambda LongC from lambda cI857 Sam7 & pUC19 & lambda cI857 Sam100 nin5
RC lambda LongD from lambda LongC
RC lambda ZAP from lambda L47.1 & pPreB & lambda LongD
RA Short J.M., Fernandez J.M., Sorge J.A., Huse W.D.;
RT "Lambda ZAP: a bacteriophage lambda expression vector with in vivo
RT excision properties";
RL Nucleic Acids Res. 16:7583-7600(1988).
XX
RN [2]
RC Uni-ZAP XR from lambda ZAP II, EcoRI-XhoI
RA Huse W.D., Hansen C.;
RT ;
RL Strategies 1:1-3(1988).
XX
CC The cI857 repressor is temperature sensitive and so is inactive
CC at 42 degrees but active at 32 degrees, allowing selection against
CC lysogeny.
CC Contains phagemid Bluescript SK-.
CC NM (lambda ZAP II)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)(linear)
CC ST ()
CC TY (phage)
CC SP (Stratagene)
CC HO (E.coli XL-1-Blue MRF')
CC CP ()
CC FN (cloning up to 10000 bp)(cloning cDNA)(cloning unidirectional)
CC FN (expression prokaryote)(antibody screening)(excision automatic)
CC SE (color blue/white)
CC PA (lambda)
CC BR (lambda ZAP Express)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. lambda
FT -> lambda cI857 Sam7
FT 1. lambda
FT -> lambda cI857 Sam100 nin5
FT 1. lambda cI857 Sam7
FT 2. pUC19
FT 3. lambda cI857 Sam100 nin5
FT -> lambda LongC
FT 1. lambda LongC
FT -> lambda LongD
FT 1. lambda L47.1
FT 2. pPreB
FT 3. lambda LongD
FT -> lambda ZAP
FT 1. lambda ZAP
FT 2. lambda gt10, S+ gene
FT -> lambda ZAP II"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cohesive end site
FT (cosL)"
FT misc_binding 0..0
FT /note="SIT BamHI"
FT CDS 0..0
FT /note="GEN bacteriophage lambda A"
FT CDS 0..0
FT /note="GEN bacteriophage lambda J"
FT CDS complement(0..0)
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT promoter complement(0..0)
FT /note="PRO E. coli lacUV5 gene"
FT promoter 0..0
FT /note="PRO bacteriophage T3"
FT misc_binding 0..0
FT /note="MCS SacI-NotI-XbaI-SpeI-EcoRI-XhoI"
FT misc_binding 0..0
FT /note="SIT unique EcoRI (in C-terminus of lacZ)"
FT promoter complement(0..0)
FT /note="PRO bacteriophage T7"
FT misc_feature 0..0
FT /note="TER helper phage initiator"
FT misc_feature 0..0
FT /note="TER helper phage terminator"
FT rep_origin 0..0
FT /note="ORI bacteriophage f1-"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI bacteriophage lambda"
FT misc_binding 0..0
FT /note="SIT XhoI"
FT misc_binding 0..0
FT /note="SIT BamHI"
FT misc_binding 0..0
FT /note="SIT PvuI"
FT CDS 0..0
FT /note="GEN bacteriophage lambda shndIII2-3"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT CDS 0..0
FT /note="GEN bacteriophage lambda cI857"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT CDS 0..0
FT /note="GEN bacteriophage lambda nin5"
FT CDS 0..0
FT /note="GEN bacteriophage lambda S100 amber (Sam100)"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cohesive end site
FT (cosL)"
XX
SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
n
//