ID   PACRP6     preliminary; circular DNA; SYN; 641 BP.
XX
AC   D10357; D00127; A00381;
XX
DT   23-JUL-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Insect baculovirus vector pAcRP6 - incomplete, EcoRV-HinfI.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-641
RC   pAcEcoRII from pUC8 & AcNPV
RC   pAcRP6, pAcRP series from pAcEcoRII
RC   pAcRP61 from pAcRP6 & linker
RC   plasmid from pAcEcoRII & linker
RC   pAcYM1 from plasmid & pAcRP61
RA   Matsuura Y., Possee R.D., Overton H.A., Bishop D.H.;
RT   "Baculovirus expression vectors: the requirements for higb level
RT   expression of proteins, including glycoproteins";
RL   J. Gen. Virol. 68:1233-1250(1987).
XX
CC   Data have been obtained with recombinant baculoviruses which
CC   indicate that the level of expression of a foreign protein that
CC   replaces the AcNPV polyhedrin gene is related to the representation
CC   of the immediate 5' upstream sequence of that gene.
CC   NCBI gi: 220955
CC   NM (pAcRP6)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (virus)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA (Autographa californica nuclear polyhedrosis virus AcNPV)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 BamHI 2665bp 241..241
FT                   fill in
FT                   EcoRI linker 6bp gaattc
FT                   2. AcNPV EcoRI-EcoRI 7300bp, polyhedrin gene
FT                   -> pAcEcoRII 9971bp
FT                   1. pAcEcoRII remove 170bp ATG..+170
FT                   BamHI linker 6bp ggatcc
FT                   -> pAcRP6 9807bp"
FT   misc_feature    49..49
FT                   /note="AcNPV polyhedrin mRNA start site"
FT   polyA_signal    430..435
FT                   /note="PLA"
XX
SQ   Sequence 641 BP; 190 A; 108 C; 113 G; 230 T; 0 other;
     tgatatcatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt
     tttcgtaaca gttttgtaat aaaaaaaccc cggatccggt tattagtaca gcatttatta
     agcgctagat tctgtgcgtt gttgatttac agacaattgt tgtacgtatt ttaataattc
     attaaattta taatctttag ggtggtatgt tagagcgaaa atcaaatgat tttcagcgtc
     tttatatctg aatttaaata ttaaatcctc aatagatttg taaaataggt ttcgattagt
     tcaaacaagg gttgtttttc cgaaccgatg actgaactat ctaatgaatt ttcgctcaac
     gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt
     ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca
     tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc ttggacatat
     ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg
     ttagaagttg cttccgaaga cgattttgcc atagccacac g
//