ID   PBTI1      preliminary; circular DNA; SYN; 11000 BP.
XX
AC   ATCC37087;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli cosmid vector pBTI-1 - incomplete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBTI-1 from MUA-10 & YEp13
RC   pBTI-5 from pBTI-1
RC   pBTI-6 from MUA-3 & pACYC184
RC   pBTI-7, pBTI-8 from pBTI-6
RC   pBTI-9 from pBTI-8
RC   pBTI-10 from pBTI-6 & YRp7
RA   Morris D.W., Noti J.D., Osborne F.A., Szalay A.A.;
RT   "Plasmid vectors capable of transferring large DNA fragments to
RT   yeast";
RL   DNA 1:27-36(1981).
XX
RN   [2]
RC   pYF40 from pBR313 & S.cerevisiae HIS3 gene
RC   pYF36 from pBR313 & S.cerevisiae LEU2 gene
RC   pYF86 from pBR322 & p82-6B, yeast 2 micron
RC   pYF84, pYF85 from pYF86
RC   pYF87 from pYF86 & pYF40
RC   pYF88 from pYF84 & pYF40
RC   pYF92 from pYF85 & pYF40
RC   pYF81 from pYF36 & p82-6B
RC   pYF90 from pYF81 & pYF88
RC   pYF91 from pYF90
RA   Storms R.K., McNeil J.B., Khandekar P.S., An J., Parker J.,
RA   Friesen J.D.;
RT   "Chimeric plasmids for cloning of deoxyribonucleic acid sequences
RT   in Saccharomyces cerevisiae";
RL   J. Bacteriol. 140:73-82(1979).
XX
CC   A Saccharomyces cerevisiae/E.coli cosmid shuttle vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pBTI-1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli JA300)
CC   HO (E.coli JA221)(Saccharomyces cerevisiae SHY2)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (YEp13)(MUA-10)
CC   BR (pBTI-5)
CC   OF (pBTIK25)(pBTIK39)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. MUA-10 PstI-PstI 350bp, lambda cos
FT                   2. YEp13 PstI 10639bp, yeast 2 micron 2657..2657
FT                   -> pBTI-1 11000bp [unique BamHI]"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_feature    0..0
FT                   /note="phage lambda cos fragment"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
FT   misc_binding    0..0
FT                   /note="SIT HpaI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT HpaI"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_feature    0..0
FT                   /note="yeast 2 micron"
FT   misc_binding    0..0
FT                   /note="SIT HpaI"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//