ID   PBTI10     preliminary; circular DNA; SYN; 7750 BP.
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AC   ATCC37090;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli cosmid vector pBTI-10 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBTI-1 from MUA-10 & YEp13
RC   pBTI-5 from pBTI-1
RC   pBTI-6 from MUA-3 & pACYC184
RC   pBTI-7, pBTI-8 from pBTI-6
RC   pBTI-9 from pBTI-8
RC   pBTI-10 from pBTI-6 & YRp7
RA   Morris D.W., Noti J.D., Osborne F.A., Szalay A.A.;
RT   "Plasmid vectors capable of transferring large DNA fragments to
RT   yeast";
RL   DNA 1:27-36(1981).
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CC   A Saccharomyces cerevisiae/E.coli cosmid shuttle vector.
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pBTI-10)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae RH218)(E.coli)
CC   HO (E.coli JA300)(Saccharomyces cerevisiae SHY2)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBTI-6)(YRp7)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. MUA-3 4695bp
FT                   2. pACYC184 EcoRI-HindIII 1523bp 2..1525
FT                   -> pBTI-6 6218bp [unique EcoRI, PstI, XbaI]
FT                   1. pBTI-6 EcoRI 6300bp 2..2
FT                   2. YRp7 EcoRI-EcoRI 1454bp 2..1456, ARS1/TRP1 gene
FT                   -> pBTI-10 7750bp [unique BamHI, SalI, in tet gene]"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   rep_origin      0..0
FT                   /note="ORI yeast ARS1"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI-HindIII-BamHI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_feature    0..0
FT                   /note="phage lambda cos fragment"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//