ID   PBTI9      preliminary; circular DNA; SYN; 10200 BP.
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AC   ATCC37089;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli cosmid vector pBTI-9 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBTI-1 from MUA-10 & YEp13
RC   pBTI-5 from pBTI-1
RC   pBTI-6 from MUA-3 & pACYC184
RC   pBTI-7, pBTI-8 from pBTI-6
RC   pBTI-9 from pBTI-8
RC   pBTI-10 from pBTI-6 & YRp7
RA   Morris D.W., Noti J.D., Osborne F.A., Szalay A.A.;
RT   "Plasmid vectors capable of transferring large DNA fragments to
RT   yeast";
RL   DNA 1:27-36(1981).
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CC   A Saccharomyces cerevisiae/E.coli cosmid shuttle vector.
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pBTI-9)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (E.coli JA221)(Saccharomyces cerevisiae SHY2)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBTI-8 from pBTI-6 from MUA-3 and pACYC184)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. MUA-3 4695bp
FT                   2. pACYC184 EcoRI-HindIII 1523bp 2..1525
FT                   -> pBTI-6 6218bp [unique EcoRI, PstI, XbaI]
FT                   1. pBTI-6 EcoRI 6218bp, pACYC184 2..2
FT                   2. YEp13 EcoRI-EcoRI 6400bp, LEU2 gene
FT                   -> pBTI-8 12600bp
FT                   1. pBTI-8 remove XbaI-XbaI 2400bp, LEU2 gene
FT                   -> pBTI-9 10200bp"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_feature    0..0
FT                   /note="yeast 2 micron"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI-HindIII-BamHI"
FT   misc_feature    0..0
FT                   /note="phage lambda cos fragment"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//