ID   PCOPNEO    preliminary; circular DNA; SYN; 6900 BP.
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AC   ATCC37409;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Drosophila/E.coli plasmid vector pcopneo - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pcopneo from pAT153 & pSV2-neo & linker & cDm5002, copia
RC   plasmid from pUC8 & pHT1, hsp70 promoter
RC   plasmid2 from ppi25.7wc, P element
RC   plasmid3 from plasmid1 & plasmid2
RC   pNHP from plasmid3 & pcopneo & linker
RA   Rio D.C., Rubin G.M.;
RT   "Transformation of cultured Drosophila melanogaster cells with a
RT   dominant selectable marker";
RL   Mol. Cell. Biol. 5:1833-1838(1985).
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RN   [2]
RC   Df(3R)Antp series from ANT-C region
RC   Xa, Xb from ANT-C region
RC   Eb, Ec, Ed, Ee from ANT-C region
RC   [cDm5002 from copia gene]
RA   Lewis R.A., Kaufman T.C., Denell R.E., Tallerico P.;
RT   "Genetic analysis of the Antennapedia gene complex (ANT-C) and
RT   adjacent chromosomal regions of Drosophila melanogaster II polytene
RT   chromosome segments 84B-D";
RL   Genetics 95:367-381(1980).
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RN   [3]
RC   ptk/delta3'-1.13 from HSV tk gene
RC   pXTK1 from pML-RIIG & ptk/delta3'-1.13, HSV tk gene
RC   pXTK10 from pXTK1 & linker
RC   pXTK12 from pXTK1 & pXls11 & linker
RC   pHT1 from pXTK10 & p132E3, hsp70 promoter
RC   pHT2 from pXTK10 & SV40 & linker & p132E3, hsp70 gene
RC   pHT3 from pHT1
RC   pHT4 from pHT1 & p56H8, hsp70 gene
RC   pHTdelta5'-44 from pHT4 & pXTK12
RC   pHT5 from pHT1 & linker & pHTdelta5'-44
RC   pHT6, pHT7 from pHT5
RC   pF1 from p56H8 & linker & pHT1
RC   pF2 from pML & p56H8 & linker
RC   pF3 from p132E3 & pML & pHT1
RC   pH1 from pML & p56H8 & SV40 & linker
RC   pH2 from pML & SV40 & linker & p132E3, hsp70 gene
RA   Pelham H.R.;
RT   "A regulatory upstream promoter element in the Drosophila hsp70
RT   heat shock gene";
RL   Cell 30:517-528(1982).
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RN   [4]
RC   pXls11 from X. laevis 5S gene
RA   Peterson R.C., Doering J.L., Brown D.D.;
RT   "Characterization of two Xenopus somatic 5S DNAs and one minor
RT   oocyte-specific 5S DNA";
RL   Cell 20:131-141(1980).
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CC   Contains dominant selectable marker (G418) for transformation of
CC   cultured Drosophila cells.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pcopneo)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(Drosophila melanogaster)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. D. melanogaster, copia gene
FT                   -> cDm5002
FT                   1. pSV2-neo HindIII-BamHI 2341bp 752..3093, neo gene
FT                   2. pAT153 BamHI-EcoRI 3281bp 376..3657
FT                   3. cDm5002 EcoRI-ApaI 1600bp, copia LTR
FT                   :T4 DNA polymerase
FT                   :HindIII linker 8bp naagcttn
FT                   -> pcopneo 6900bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-HindIII-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO Drosophila copia gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT Drosophila G418 resistance gene (G418)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//