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ID PHSG250 preliminary; circular DNA; SYN; 1700 BP.
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AC ATCC37299;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli cosmid vector pHSG250 - incomplete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pHSG306 from ColE1 & Tn5
RC pHSG415s from pHSG415
RC pHSG418 from pHSG415s
RC pHSG425 from pHSG418
RC pHSG429 from pHSG425 & pHSG422
RC pHSG124 from ColE1 & Tn3 & pHSG1
RC pHSG402 from pHSG124
RC pHSG231 from pHSG402
RC pHSG232 from pHSG231
RC pHSG255 from pHSG232 & pHSG429
RC pHSG250 from pHSG255
RC pHSG262 from pHSG255
RC pHSG415h from pHSG415s
RC pHSG413h from pHSG415h
RC pHSG439 from pHSG413h & pHSG262
RC pBRG418 from pBR322 & pAG60, Tn5 kan/HSV tk gene promoter/polyA
RC pHSG272 or pHS272 from pBRG418 & pHSG262
RC pHSG274 from pBRG418 & pHSG255 & pUC8
RC pHSG591 from pNO1523, lambda cos
RC pHSG660 from pNO1523
RA Brady G., Jantzen H.M., Bernard H.U., Brown R., Schutz G.,
RA Hashimoto-Gotoh T.;
RT "New cosmid vectors developed for eukaryotic DNA cloning";
RL Gene 27:223-232(1984).
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RN [2]
RC pDMS630 or 6-30 from ColE1 & Tn3
RA Inselburg J.;
RT "Isolation, mapping, and exanmination of effects of TnA insertions
RT in ColE1 plasmids";
RL J. Bacteriol. 129:482-491(1977).
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RN [3]
RC pSC134 from pSC101 & ColE1
RA Timmis K., Cabello F., Cohen S.N.;
RT "Utilization of two distinct modes of replication by a hybrid
RT plasmid constructed in vitro from separate replicons";
RL Proc. Natl. Acad. Sci. U.S.A. 71:4556-4560(1974).
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RN [4]
RC ColE1-Tn5 from ColE1 & Tn5
RA Berg D.E.;
RT "Insertion and excision of the transposable kanamycin resistance
RT determinant Tn5";
RL DNA Insertion Element, Plasmids, and Episomes 0:205-212(1977).
RL Bukhari A.I., Shapiro J.A., Adhya S.L.;
RL Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
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CC A cosmid vector suitable for cloning large DNA fragments.
CC Medium is 1065 LB medium.
CC NM (pHSG250)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (cosmid)
CC SP (ATCC)
CC HO (E.coli SK1592)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pSC101 9263bp, ori/tet gene
FT mutagenesis
FT -> pHSG1
FT 1. E. coli
FT -> R1
FT 1. R1
FT -> R1drd-19
FT 1. ColE1 6646bp
FT transposition
FT 2. R1drd-19, Tn3 amp gene
FT -> pDMS630
FT 1. pHSG1 EcoRI 9263bp 9262..9262
FT 2. pDMS630 EcoRI 6646bp 6645..6645
FT -> pHSG124 15909bp
FT 1. pHSG124 remove BamHI-BamHI, pSC101 ori
FT \ pSC101 376 to R1drd-19 Tn3 amp gene
FT -> pHSG401
FT 1. pSC101 EcoRI 9263bp 9262..9262
FT 2. R6-5 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT -> pSC105 16163bp
FT 1. pSC105 EcoRI-EcoRI 6900bp, Tn903 kan gene/#V00359
FT 2. pVH51 EcoRI 3847bp 3846..3846
FT -> pML21 10747bp
FT 1. pHSG401
FT 2. pML21 10747bp
FT -> pHSG408
FT 1. pHSG408
FT -> pHSG410
FT 1. pHSG410
FT -> pHSG412
FT 1. pHSG412
FT 2. pACYC184 4245bp
FT -> pHSG415
FT 1. pHSG415
FT -> pHSG415s
FT 1. pHSG415s
FT -> pHSG418
FT 1. pHSG418 remove small PstI-PstI
FT -> pHSG425
FT 1. pHSG415
FT 2. pHC79 BglII-BglII 1719bp 2111..3830, lambda cos
FT -> pHSG422
FT 1. pHSG425 BstEII
FT 2. pHSG422 BstEII-BstEII, amp gene/lambda cos
FT -> pHSG429
FT 1. pHSG124 remove small BamHI-BamHI
FT -> pHSG402 [unique EcoRI, BamHI]
FT 1. pHSG402 remove small HaeII-HaeII
FT -> pHSG231 [unique EcoRI, BamHI]
FT 1. pHSG231 EcoRI
FT 2. pivx EcoRI-EcoRI, MCS
FT EcoRI-ClaI-HindIII-XbaI-BglII-PstI-BamHI-EcoRI
FT -> plasmid
FT 1. plasmid remove small BamHI-BamHI, MCS
FT -> pHSG232
FT 1. pHSG232 BamHI
FT T4 DNA polymerase
FT HaeII-HaeII
FT 2. pHSG429 HaeII-PvuII, kan gene/lambda cos
FT -> pHSG255 [unique EcoRI, BamHI, HincII]
FT 1. pHSG255 HaeII-HaeII 1430bp, kan gene/#V00359
FT -> pHSG250 1700bp"
FT misc_binding 0..0
FT /note="SIT unique EcoRI-BamHI-HincII"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="GEN E. coli colicin E1 immunity gene"
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SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
n
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