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ID PJRD215 preliminary; circular DNA; SYN; 10200 BP.
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AC M16198; ATCC37533;
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DT 02-APR-1988 (Rel. 3, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Broad host range/E.coli cosmid vector pJRD215 - KmR-cos region.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-2549
RC pJRD185 from pJRD184
RC pJRD186 from pIC20R & pJRD185
RC pEMBLcos from pEMBL8 & MUA-10, lambda cos
RC RSF1010KmR or RSF1010Kan from RSF1010 & pKC7
RC pJRD203 from RSF1010KmR or RSF1010Kan & pEMBLcos
RC pJRD204 from pJRD203
RC pJRD209 from pJRD203
RC pJRD210 from pJRD204
RC pJRD212 from pJRD209 & pJRD210
RC pJRD215 from pJRD212 & pJRD186
RA Davison J., Heusterspreute M., Chevalier N., Ha-Thi V., Brunel F.;
RT "Vectors with restriction site banks: V. pPJRD215, a wide-host-
RT range cosmid vector with multiple cloning sites";
RL Gene 51:275-280(1987).
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RN [2]
RC pJRD215
RA Davison J.;
RT ;
RL Submitted (01-JUL-1987) to GenBank on computer-readable media by:
RL Davison J., .
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CC Broad host range, multiple cloning site cosmid that can be
CC transferred by conjugation.
CC Medium is 1236 LB plus kanamycin.
CC NCBI gi: 208692
CC NM (pJRD215)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (cosmid)
CC SP (ATCC)
CC HO (E.coli MM294)(E.coli)(broad host range)
CC CP ()
CC FN (cloning)(shuttle wide-host-range)(restriction site bank)
CC SE ()
CC PA (pBR327)(lambda)(pEMBL8)(MUA-10)(pJRD186)(pBR322)(Tn5m)
CC BR (pJRD158)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
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FT misc_feature 0..0
FT /note="1. pJRD184 remove small BclI-BglII 5bp
FT \ 1605..1610, 3898bp
FT -> pJRD185 3898bp
FT 1. pIC20R HindIII-KpnI 43bp 264..307, MCS
FT 2. pJRD185 remove HindIII-KpnI 1298bp 30..1328, 2600bp
FT -> pJRD186 2600bp
FT 1. pEMBL8+ PstI 3939bp 255..255
FT 2. MUA-10 PstI-PstI 403bp, lambda cos
FT -> pEMBLcos 4342bp
FT 1. RSF1010 remove EcoRI-PstI 904bp 7773..8677,
FT \ sulf/7780bp
FT 2. pKC7 EcoRI-PstI 1447bp 5834..5835..1446, Tn5 kan
FT -> RSF1010KmR 9227bp
FT 1. RSF1010KmR remove small EcoRI-HindIII 8677..?
FT 2. pEMBLcos EcoRI-HindIII 433bp,
FT \ pEMBL8+ 229..-..259 MCS/lambda cos
FT -> pJRD203 9100bp
FT \ [unique BglII-HindIII-SalI-BamHI-EcoRI-HpaI-SacI]
FT 1. pJRD203 remove small EcoRI-HpaI, 9100bp
FT 2. pBR322 EcoRI-EcoRV 189bp 4360..4361..188,
FT \ tet promoter/185bp
FT -> pJRD204 9000bp [unique ClaI, no HpaI, 2 HindIII]
FT 1. pJRD203 HindIII 9100bp
FT fill in
FT -> pJRD209 9100bp [unique NheI]
FT 1. pJRD204 AsuII 9000bp
FT Klenow
FT XhoI linker 6bp ctcgag
FT -> pJRD210 9000bp [unique XhoI]
FT 1. pJRD209 9100bp BglII-SalI
FT 2. pJRD210 remove BglII-SalI
FT -> pJRD212 9000bp [unique XhoI, NheI, HindIII]
FT 1. pJRD212 SmaI-BstEII
FT 2. pJRD212 BstEII-BamHI
FT 3. pJRD186 BamHI-PvuII 432bp, MCS
FT -> pJRD215 10200bp"
FT CDS 0..0
FT /note="ANT E. coli kanamycin resistance gene (kan);
FT fragment; from pKC7"
FT CDS 0..0
FT /note="ANT E. coli streptomycin resistance gene (str);
FT fragment; from pKC7"
FT rep_origin 0..0
FT /note="ORI bacteriophage lambda"
FT misc_binding 0..0
FT /note="SIT bacteriophage lambda cos region"
FT misc_recomb 1467..1468
FT /note="Tn5 DNA end/pEMBL8 DNA start"
FT misc_recomb 1483..1484
FT /note="pEMBL8 DNA end/MuA-10 DNA start"
FT misc_recomb 1915..1916
FT /note="MuA-10 DNA end/pEMBL8 DNA start"
FT misc_recomb 1923..1924
FT /note="pEMBL8 DNA end/pJRD186 DNA start"
FT misc_recomb 2357..2358
FT /note="pJRD186 DNA end/pEMBL8 DNA start"
FT misc_recomb 2360..2361
FT /note="pEMBL8 DNA end/pBR322 DNA start"
FT misc_binding 0..0
FT /note="MCS BamHI-KpnI-AsuII-MluI-HpaI-ApaI-NsiI-
FT NdeI-StuI-XbaI-SnaBI-SpeI-EcoRI"
FT rep_origin 0..0
FT /note="ORI E. coli IncQ plasmid RSF1010 oriT and oriV"
FT misc_binding 0..0
FT /note="SIT BstEII"
FT misc_binding 0..0
FT /note="SIT MstII"
FT misc_binding 0..0
FT /note="SIT XhoI"
FT misc_binding 0..0
FT /note="SIT BglII"
FT misc_binding 0..0
FT /note="SIT NheI"
FT misc_binding 0..0
FT /note="SIT DraI"
FT misc_binding 0..0
FT /note="SIT SalI"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT misc_binding 0..0
FT /note="SIT SacI"
FT misc_binding 0..0
FT /note="SIT ClaI"
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SQ Sequence 2549 BP; 607 A; 725 C; 655 G; 562 T; 0 other;
ctgcagttcg ggggcatgga tgcgcggata gccgctgctg gtttcctgga tgccgacgga
tttgcactgc cggtagaact ccgcgaggtc gtccagcctc aggcagcagc tgaaccaact
cgcgagggga tcgagcccgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag
gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgcctcg
aggcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct
ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag
gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
ttgatcagat cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt
tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct
tgctgtccat aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg
ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg
gggtcagcac cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt
gcgccctgag tgcttgcggc agcgtgaagc tagcttggct gcaggggggg gggggggact
tccattgttc attccacgga caaaaacaga gaaaggaaac gacagaggcc aaaaagcctc
gctttcagca cctgtcgttt cctttctttt cagagggtat tttaaataaa aacattaagt
tatgacgaag aagaacggaa acgccttaaa ccggaaaatt ttcataaata gcgaaaaccc
gcgaggtcgc cgccccgtaa cctgtcggat caccggaaag gacccgtaaa gtgataatga
ttatcatcta catatcacaa cgtgcgtgga ggccatcaaa ccacgtcaaa taatcaatta
tgacgcaggt atcgtattaa ttgatctgca tcaacttaac gtaaaaacaa cttcagacaa
tacaaatcag cgacactgaa tacggggcaa cctcatgtcc cccccccccc ctgcaggtcg
acggatcccc gggtaccata agtagaagca gcaacccaag tagctttacc agcatccggt
tcaaccagca tagtaagaat cttactggac atcggcagtt cttcgaacag tgcgccaact
accagctctt tctgcagttc attcagggca ccggagaacc tgcgtgcaat ccatcttgtt
caatcatgcg aaacgatcct catcctgtct cttgatccat ggattacgcg ttaacccggg
cccgcggatg catatgatct taaggcctag gtctagagtc tttgttttga cgccattagc
gtacgtaaca atcctcgtta aaggacaagg acctgagcgg aagtgtatcg tacagtagac
ggagtatact agtatagtct atagtccgtg gaattattat atttatctcc gacgatattc
tcatcagtga aatccagggg aattctcatg tttgacagct tatcatcgat aagctttaat
gcggtagttt atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca
atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta
tgccggtact gccgggcctc ttgcgggat
//