ID   PLAFR1     preliminary; circular DNA; SYN; 21600 BP.
XX
AC   ATCC37167;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Broad host range/E.coli cosmid vector pLAFR1 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pLAFR1 from pHC79 & pRK290
RA   Friedman A.M., Long S.R., Brown S.E., Buikema W.J., Ausubel F.M.;
RT   "Construction of a broad host range cosmid cloning vector and its
RT   use in the genetic analysis of Rhizobium mutants";
RL   Gene 18:289-296(1982).
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CC   A broad host range cosmid vector containing a relaxation complex site.
CC   (ATCC staff)
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pLAFR1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli MM294)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pHC79 BglII-BglII 1719bp 2111..3830,
FT                   \ lambda cos
FT                   2. pRK290 BglII 20000bp
FT                   -> pLAFR1 21600bp [unique EcoRI]"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SalI-BstEII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//