ID   PNO1517    preliminary; circular DNA; SYN; 8200 BP.
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AC   ATCC37094;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli cosmid vector pNO1517 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pNO1517 from lambda & pBR322
RC   pNO1523 from pNO1517
RA   Dean D.;
RT   "A plasmid cloning vector for the direct selection of strains
RT   carrying recombinant plasmids";
RL   Gene 15:99-102(1981).
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CC   A cosmid vector for positive selection of clones with inserts based on
CC   the dominance of the plasmid-coded rpsL+ gene.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pNO1517)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli MC1009)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda fus3 BamHI-BglII 3800bp,
FT                   \ cos/S12/S7/str promoter
FT                   \ lambda BamHI = 5506 22347 27973 34500 41733
FT                   \ lambda BglII = 416 22426 35712 38104 38755 38815
FT                   2. pBR322 BamHI 4361bp 376..376
FT                   -> pNO1517 8200bp [no BamHI, no BglII]"
FT   misc_binding    0..0
FT                   /note="SIT unique SmaI-HpaI-BstEII-SstII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   promoter        0..0
FT                   /note="PRO E. coli str gene"
FT   CDS             0..0
FT                   /note="GEN E. coli rpsL+ reporter gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//