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ID PPI251 preliminary; circular DNA; SYN; 8800 BP.
XX
AC X06779; V01520; X69493; ATCC37236;
XX
DT 30-SEP-1988 (Rel. 3, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Drosophila/E.coli plasmid vector ppi25.1 - incomplete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-4734
RC ppi25.1
RA O'Hare K.;
RT ;
RL Submitted (09-FEB-1988) on tape to the EMBL Data Library by:
RL O'Hare K., Department Biochemistry, Imperial College, London, UK.
XX
RN [2]
RC ppi25.1, ppi25 from pBR322 & P element
RC pS25.1 from pBR322 & Drosophila DNA
RC [ppi25.7 from pBR322 & Drosophila DNA]
RA Spradling A.C., Rubin G.M.;
RT "Transposition of cloned P elements into Drosophila germ line
RT chromosomes";
RL Science 218:341-347(1982).
XX
RN [3]
RC pry1, pry3 from p6.1 & rosy gene
RC pDm2844S8.5 from pry1
RC pm12.8 from pry1
RC pch1 from p6.1 & Drosophila chorion gene/66D region
RA Rubin G.M., Spradling A.C.;
RT "Genetic transformation of Drosophila with transposable element
RT vectors";
RL Science 218:348-353(1982).
XX
RN [4]
RP 1-4734
RC lambda pi12, lambda pi25 from Charon 28 & Drosophila P pi2
RC lambda pi30, lambda pi44 from Charon 28 & Drosophila P pi2
RC lambda S12, lambda S25 from Charon 28 & Drosophila M strain
RC lambda S30, lambda S44 from Charon 28 & Drosophila M strain
RC ppi25.1 from lambda pi25 & pBR322
RC ppi12.20 from lambda pi12 & pBR322
RC pS25.1 from lambda S25 & pBR322
RC pS12.20 from lambda S12 & pBR322
RC p6.1 from lambda & pBR322 & Drosophila white locus
RA O'Hare K., Rubin G.M.;
RT "Structures of P transposable elements and their sites of
RT insertion and excision in the Drosophila melanogaster genome";
RL Cell 34:25-35(1983).
XX
RN [5]
RC lambda m1.1, lambda m1.2 from Drosophila white locus
RC lambda pi.3, lambda pi.10 from Charon 28 & Drosophila white locus
RC lambda pi40.8 from Charon 28 & Drosophila white locus
RC lambda 6.1, lambda 6.4 from pBR322 & Drosophila white locus
RC lambda 12.3, lambda 12.4, lambda 12.6 from pBR322 & white locus
RC lambda 12.12 from pBR322 & white locus
RC p6.1 from pBR322 & lambda 6.1
RA Rubin G.M., Kidwell M.G., Bingham P.M.;
RT "The molecular basis of P-M hybrid dysgenesis: the nature of
RT induced mutations";
RL Cell 29:987-994(1982).
XX
RN [6]
RC lambda 103 from lambda & Drosophila chorion gene/66D region
RC lambda 104 from lambda & Drosophila chorion gene/66D region
RC lambda 302 from lambda & Drosophila chorion gene/66D region
RC p103.48, p103.29 from lambda 103, Drosophila chorion gene/66D region
RC p104.60, p104.41 from lambda 104, Drosophila chorion gene/66D region
RC p302.77, p302.42 from lambda 302, Drosophila chorion gene/66D region
RA Spradling A.C.;
RT "The organization and amplification of two chromosomal domains
RT containing Drosophila chorion genes";
RL Cell 27:193-201(1981).
XX
CC The sequence is from cytological location 17C of strain
CC pi2 of Drosophila melanogaster.
CC Plasmid used for P-element mediated gene transfer.
CC Contains the complete 2.9 kb P element of Drosophila melanogaster.
CC Medium is 1227 LB plus ampicillin.
CC NM (ppi25.1)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (D.melanogaster)(Drosophila)(E.coli K-12)(E.coli HB101)(E.coli)
CC CP ()
CC FN (gene transfer)(transformation)(transposable element)
CC SE ()
CC PA (Drosophila strain pi2 location 17C)(P element)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. Charon 28
FT 2. Drosophila P pi2
FT -> lambda pi25
FT 1. pBR322 BamHI 4361bp 376..376
FT 2. lambda pi25 BamHI-BamHI 2900bp, P element
FT -> ppi25.1 8800bp"
FT cellular 1..995
FT /note="from Drosophila strain pi2, location 17C"
FT misc_feature 812..812
FT /note="A in Canton S"
FT misc_feature 902..902
FT /note="A in Canton S"
FT misc_feature 938..938
FT /note="T in Canton S"
FT repeat_region 988..995
FT /note="RPT direct repeat 1"
FT transposon 996..3902
FT /note="TRN Drosophila P element"
FT repeat_region 3903..3910
FT /note="RPT direct repeat 1"
FT cellular 3911..4734
FT /note="from Drosophila strain pi2, location 17C"
FT misc_feature 3996..3996
FT /note="ADP AAAAAAAG inserted in Canton S"
FT misc_feature 4092..4092
FT /note="G in Canton S"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_binding 0..0
FT /note="SIT unique XhoI"
FT misc_binding 0..0
FT /note="SIT unique SacI"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
XX
SQ Sequence 4734 BP; 1501 A; 878 C; 909 G; 1446 T; 0 other;
ggatcccaat ccgcatcgaa taagacgcaa acttttgcat cgtatattga aaagatgcgc
tatctccatg gcaaagtttt tgcggtcgtc gcagacaaaa gacgcgtgtt cccttttaaa
gtttcattaa tcaatggctc gggtggtgga tgtggatgtg gctgtagcta ttgatgtagc
tggaggggtt ctgccacagc tccggctata gcttttatta tttcaattgc ggaacggtgc
ccggggctca aaaatatgag gcatcattta tcaggccgcc attccaccac tccactacgt
cataccattc cgttctggtg cggattggct gtctgggctg tggatttcaa attggcagca
acaatgccgt gattaatggc cgtcaaagcg aaagagtcaa caaatgtcaa gcgacaaact
gcaattggca attggaggga gggggggtgg tggtggtggt aaagaggacc aaatcccaaa
aaaccccata ccataacata taccatatac catactatac catggtagga atcccttggc
ccgcgatgaa atgaatgcca acacaagtgc gggttaattt tttcaaatgt ttgttaatta
catttattgt ttccttttgt ttttgcattt gcatttgttt ttatgttttt ttttctctcg
tttctcttta gttttgattt ctttcacttg caatcgttct ggctgctttc attattactg
attgccggcc aaaactcgga ttatggtttg tttttgtaat gtatatatat gcacgtatat
ataagtctaa gcgggtttat cttacacttt agatattttt gatttaactt tatgcctgct
ctctaacaat acaatacaaa aaaaatattg gataatcgct cttacttatt tccgattatt
agatattatt acgatattta acatagtttc ggtaattcga tagataggta gtattgttta
tcgctcgtag atgatagact gttgcttata cacaccatga tgaaataaca taaggtggtc
ccgtcgaaag ccgaagctta ccgaagtata cacttaaatt cagtgcacgt ttgcttgttg
agaggaaagg ttgtgtgcgg acgaattttt ttttgaaaac attaaccctt acgtggaata
aaaaaaaatg aaatattgca aattttgctg caaagctgtg actggagtaa aattaattca
cgtgccgaag tgtgctatta agagaaaatt gtgggagcag agccttgggt gcagccttgg
tgaaaactcc caaatttgtg atacccactt taatgattcg cagtggaagg ctgcacctgc
aaaaggtcag acatttaaaa ggaggcgact caacgcagat gccgtaccta gtaaagtgat
agagcctgaa ccagaaaaga taaaagaagg ctataccagt gggagtacac aaacagagta
agtttgaata gtaaaaaaaa tcatttatgt aaacaataac gtgactgtgc gttaggtcct
gttcattgtt taatgaaaat aagagcttga gggaaaaaat tcgtactttg gagtacgaaa
tgcgtcgttt agagcagcag ctgagggagt ctcaacagtt ggaggagtct ctacgcaaaa
tcttcacgga cacgcagata cggatactga agaatggtgg acaaagagct acgttcaatt
ccgacgacat ttctacagct atttgtctcc acaccgcagg ccctcgagcg tataaccatc
tgtacaaaaa aggatttcct ttgcccagtc gtacgacttt gtacagatgg ttatcagatg
tggacataaa aagaggatgt ttggatgtgg tcatagacct aatggacagt gatggagttg
atgacgccga caagctttgc gtactcgctt tcgacgagat gaaggtcgct gctgccttcg
agtatgacag ctctgctgat attgtttacg agccaagcga ctatgtccaa ctggctattg
ttcgtggtct aaaaaaatcg tggaagcagc cagttttttt cgattttaat acccgaatgg
acccggatac tcttaacaat atattaagga aactgcatag gaaaggatat ttagtagttg
ctattgtatc cgatttaggt accggaaacc aaaagctatg gacagagctc ggtatatcag
aatgtaagtt tcgtatatta caaaaatcag ataatccttg aaattccatt ttttagcaaa
aacctggttt agccatcctg cagatgacca tttaaagatt ttcgtttttt cggatacgcc
acatttaatt aagttagtcc gtaaccacta tgtggattcc ggattaacaa taaatgggaa
aaaattaaca aaaaaaacaa ttcaggaggc acttcatctt tgcaacaagt ccgatctgtc
tatcctcttt aaaattaatg aaaatcacat taatgttcga tcgctcgcaa aacagaaggt
taaattggct acccagctgt tttcgaatac caccgctagc tcgatcagac gctgctattc
attggggtat gacattgaaa atgccaccga aactgcggac ttcttcaaat tgatgaatga
ttggttcgac atttttaatt ctaaattgtc cacatccaat tgcattgagt gctcgcaacc
ttatggcaag cagttggata tacagaatga tattttgaat cgaatgtcgg aaattatgcg
aacaggaatt ctggataaac ccaaaaggct cccatttcaa aaaggtatca ttgtgaataa
tgcttcgctt gatggcttgt ataaatattt gcaagaaaac ttcagtatgc aatacatatt
aacaagccgt ctcaaccaag acattgtgga gcattttttt ggcagcatgc gatcgagagg
tggacaattc gaccatccca ctccactgca gtttaagtat aggttaagaa aatatataat
aggtatgaca aatttaaaag aatgcgtaaa caaaaatgta attccatgat ttataattgt
ttaatgttta gctatatgtt tcaggaaagt ttcagttgag aatgtaggta gttatgtgct
gtctattgtg ttttgtcttt tatctgtttc ttttcatttt attatttaat cattatcctt
ttgcttatcc agccaggaat acagaaatgt taagaaattc gggaaatatc gaagaggaca
actctgaaag ctggcttaat ttagatttca gttctaaaga aaacgaaaat aaaagtaaag
atgatgagcc tgtcgatgat gagcctgtcg atgagatgtt aagcaatata gatttcaccg
aaatggatga gttgacggag gatgcgatgg aatatatcgc gggctatgtc attaaaaaat
tgagaatcag tgacaaagta aaagaaaatt tgacatttac atacgtcgac gaggtgtctc
acggcggact tattaagccg tccgaaaaat ttcaagagaa gttaaaagag ctagaatgta
tttttttgca ttatacaaat aataataatt ttgaaattac aaataatgta aaggaaaaat
taatattagc agcgcgaaac gtcgatgttg ataaacaagt aaaatctttt tattttaaaa
ttagaatata ttttagaatt aagtacttca acaaaaaaat tgaaattaaa aatcaaaaac
aaaagttaat tggaaactcc aaattattaa aaataaaact ttaaaaataa tttcgtctaa
ttaatattat gagttaattc aaaccccacg gacatgctaa gggttaatca acaatcatat
cgctgtctca ctcagactca atacgacact cagaatacta ttcctttcac tcgcacttat
tgcaagcata cgttaagtgg atgtctcttg ccgacgggac caccttatgt tatttcatca
tgatacacac actttggttt ctcccgtact tagtatattc gctagctcct ttcgaacgct
aattacgcat attgattagt gttacattag attgcaaaaa aaaaaaggat taataaatac
atccatattg taactagttc gcttggttat agatatacac acgcacgcac acaaatcctt
aactttacat ccacctatct taggcgcttt acatctaacg cgctggatca tcgtggatct
ggccatcata cctcatctac aatccatcta gaaatcatcc agctccagct acttctatac
aacgttcgcc gtggaaagaa cattctttag cctcgcacta aactaaactt aaataataat
caacaacgtt gctccaacag acttaaaatg gaattagaac aaaaatcatg tgtgcttaag
tcgtgtgcag aatgtctcaa tatttacaca tcaaagttca cttttgccaa ttgccctcta
gctcctctag ctcctcatca tctatctctc ttcatctgac tctactctac gatctgcggc
tcttcggatt cgaaggacac atcgaaaatc gaacagtgat cggtggggat gtttagctct
ctcgatttcg cagtccacaa aatccgattc tctccaccac cagccgccct tgtcattcat
tccacattca tcagtttagt ccttcgttta tttttttttt tttaactttt tggcactgct
ttgaatttct tttggcaccg cacgcttcga gcgtgaaaat aaaattattt atgcatatac
atagataaat acgaatatat ctatatggag tatgtaaaaa aaaaaaaagg atcc
//