ID   PPUC29     preliminary; circular DNA; SYN; 14200 BP.
XX
AC   ATCC37217;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Anacystis/E.coli cosmid vector pPUC29 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pUC104 from pUC1 & pACYC184
RC   pUC29 from pUC104
RC   pPUC29 from pUC104 & lambda, cos
RC   pTH225 from pPUC29 & A.nidulans R-2 met gene
RC   pBHT187 from pACYC184 & Tn901 & A.nidulans R-2 met gene
RA   Tandeau De Marsac N., Borrias W.E., Kuhlemeier C.J., Castets A.M.,
RA   Van Arkel G.A., Van Den Hondel C.A.;
RT   "A new approach for molecular cloning in cyanobacteria: cloning of
RT   an Anacystis nidulans met gene using a Tn901-induced mutant";
RL   Gene 20:111-119(1982).
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CC   The ampR gene is carried on a 1.7 kb fragment of Tn901 which has lost
CC   the ability to transpose.
CC   A cosmid shuttle vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pPUC29)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli 803)(Anacystis nidulans)(E.coli)(E.coli K802 recA-)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pACYC184 BamHI 4245bp 1871..1871, cat gene
FT                   2. pUC1 BamHI-BamHI 1700bp, Tn901 amp gene
FT                   -> pUC104 6000bp
FT                   1. pUC104 BglII 6000bp 1871..1871, Tn901 amp gene
FT                   2. pHC79 BglII-BglII 1719bp 2111..3830, lambda cos
FT                   -> pPUC29 14200bp [unique EcoRI in cat gene]"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//