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ID PSW272 preliminary; circular DNA; SYN; 8700 BP.
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AC ATCC39609;
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DT 01-JUL-1993 (Rel. 7, Created)
DT 01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE Vertebrate/E.coli virus vector pSW272 - incomplete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pSW253 from pBR322 & REV-A
RC pSW254 from pSW253
RC plasmid from pBR322 & pSW253 & REV-A provirus
RC pSW279 from plasmid & pSW210
RC pSW283 from pSW253 & pSW210
RC pSW272 from pSW227 & linker
RC SW272 from pSW272 & SNV-TK
RC SW279 from pSW279 & SNV-TK
RC SW283 from pSW283 & SNV-TK
RA Watanabe S., Temin H.M.;
RT "Construction of a helper cell line for avian
RT reticuloendotheliosis virus cloning vectors";
RL Mol. Cell. Biol. 3:2241-2249(1983).
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RN [2]
RC pTK23adeltadeltaterR from pSNV-60B & HSV TKdeltaterR
RC pSW222 from pTK23adeltadeltaterR
RC pSW210 from pBR322 & pSNV-60B & linker
RC pSW227 from pSW210 & pSW222
RC pSW226, pSW249, pSW250, pSW251 from pSW227
RA Watanabe S., Temin H.M.;
RT "Encapsidation sequences for spleen necrosis virus, an avian
RT retrovirus, are between the 5' long terminal repeat and the start
RT of the gag gene";
RL Proc. Natl. Acad. Sci. U.S.A. 79:5986-5990(1982).
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RN [3]
RC pSW272 from pSW227 & linker
RA Temin H.M.;
RT ;
RL Unpublished (1987).
RL U.S. Patent No. 4,650,764 dated Mar. 17, 1987.
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RN [4]
RC pTK2, pTK9, pTK19, pTK22, pTK23 from p60BSal & pTK1, HSV tk gene
RC pTKdeltater(RP) from pTK1
RC pTK2deltadeltater(RP) from pTKdeltater(RP) & pTK2
RC SNV TK2deltadeltater(R) from SNV & pTK2deltadeltater(RP)
RA Shimotohno K., Temin H.M.;
RT "Formation of infectious progeny virus after insertion of herpes
RT simplex thymidine kinase gene into DNA of an avian retrovirus";
RL Cell 26:67-77(1981).
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RN [5]
RC pREV-T S/H 5', pREV-T Hp/S 3' from pBR322 & REV & SNV
RA Chen I.S., Temin H.M.;
RT "Substitution of 5' helper virus sequences into non-rel portion of
RT reticuloendotheliosis virus strain T suppresses transformation of
RT chicken spleen cells";
RL Cell 31:111-120(1982).
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RN [6]
RC pGT2 from SNV & HSV tk gene
RC pGT6 from SNV & HSV tk gene
RA Tarpley W.G.;
RT ;
RL Unpublished (1984).
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RN [7]
RC p60BSal from pBR322 & SNV
RA O'Rear J.J., Temin H.M.;
RT "Mapping of alterations in non-infectious proviruses of spleen
RT necrosis virus";
RL J. Virol. 39:138-149(1981).
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CC This retrovirus vector is itself replication incompetent and is
CC designed to be used with a helper cell line (ATCC CRL 8468). [3]
CC Medium is 1227 LB plus ampicillin.
CC NM (pSW272)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (virus)
CC SP (ATCC)
CC HO (E.coli HB101)(vertebrate cells)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (avian reticuloendotheliosis virus)(spleen necrosis virus SNV)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pSNV-60B remove BglII-BglII 4800bp
FT [1900..6700]
FT 2. HSV BglII-BglII, TK gene with no polyA/TKdeltaterR
FT -> pTK23adeltadeltaterR
FT 1. pTK23adeltadeltaterR remove XbaI-XbaI 900bp
FT [1020..1950]
FT -> pSW222 [SNV-TKdeltaterR]
FT 1. pBR322 SalI
FT 2. pSNV-60B remove SalI-AvrII 860..7700
FT :EcoRI linker
FT -> pSW210 [SNV LTR]
FT 1. pSW210 SalI
FT 2. pSW222 SalI-SalI, SNV-TKdeltaterR
FT -> pSW227
FT 1. pSW227 XbaI
FT BAL31 nuclease
FT XbaI linker:XbaI linker
FT -> pSW272"
FT misc_binding 0..0
FT /note="SIT unique XbaI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT promoter 0..0
FT /note="PRO spleen necrosis virus (SNV) LTR;
FT retrovirus"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="GEN vertebrate thymidine kinase gene (TK+)"
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SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
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