ID   PVK102     preliminary; circular DNA; SYN; 23000 BP.
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AC   ATCC37158;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Broad host range/E.coli cosmid vector pVK102 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pVK100 from pRK290 & pHK17
RC   pVK101 from pVK100
RC   pVK102 from pVK100
RC   pVK200 series from pVK102 & pTiA6
RC   pVK261 from pVK102 & pTiA6
RA   Knauf V.C., Nester E.W.;
RT   "Wide host range cloning vectors: a cosmid clone bank of an
RT   Agrobacterium Ti plasmid";
RL   Plasmid 8:45-54(1982).
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CC   Can be mated into Agrobacterium using helper plasmid pRK2013
CC   (ATCC 37159).
CC   Independent replicon enables cloned insert to complement in trans.
CC   A broad host range plasmid vector.
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pVK102)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(broad host range)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pVK100 EcoRI 23000bp
FT                   T4 DNA polymerase
FT                   -> pVK102 23000bp [no EcoRI]"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-XhoI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2"
FT   CDS             0..0
FT                   /note="ANT Pseudomonas kanamycin resistance gene
FT                   (kan)"
FT   CDS             0..0
FT                   /note="ANT Pseudomonas tetracycline resistance gene
FT                   (tet)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//