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ID PW8 preliminary; circular DNA; SYN; 147 BP.
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AC X05401;
XX
DT 06-JUL-1989 (Rel. 4, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Drosophila/E.coli plasmid vector pW8 - incomplete, MCS.
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KW cloning vector; Drosophila melanogaster; polylinker; P element;
KW germ line transformation; hsp70-white.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-147
RC pW1 from pUC18 & pWP-2
RC pW2 from pW1
RC pW3 from pW2
RC pW4 from pW3 & pHSL
RC car4.3 from Carnegie 4 & linker
RC pW5, pW6 from pW4 & car4.3
RC pW7 from pW5 & linker
RC pW8 from pW7 & linker
RC pWAc from pW5 & p75RH, indirect flight muscle specific actin gene
RA Klemenz R., Weber U., Gehring W.J.;
RT "The white gene as a marker in a new P-element vector for gene
RT transfer in Drosophila";
RL Nucleic Acids Res. 15:3947-3959(1987).
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RN [2]
RC delta1 from Charon 4 & Drosophila DNA
RC M363, M407, M410, M505, M510, M512, M558, M570 from delta1, white
RA Goldberg M.L., Paro R., Gehring W.J.;
RT "Molecular cloning of the white locus region of Drosophila
RT melanogaster usign a large transposable element";
RL EMBO J. 1:93-98(1982).
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RN [3]
RC p6.3 from p6.1 & pUC9
RC pWP-2 from phage M558 & p6.3, P element
RA Gehring W.J., Klemenz R., Weber U., Kloter U.;
RT "Functional analysis of the white+ gene of Drosophila by
RT P-factor-mediated transformation";
RL EMBO J. 3:2077-2085(1984).
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RN [4]
RC pHSPr from pAT153 & p56H8, hsp70 gene
RC pHSL from pAT153 & p56H8, hsp70 gene
RC pAH from sAC-1 & pHSPr
RC pAHdelta from pAH
RC pCAH from pAHdelta & Carnegie 20 or car20, rosy gene/P element
RC pCadh from sAC-1 & Carnegie 20 or car20, rosy gene/P element
RC sAC-1delta from sAC-1
RC pHSLdelta1, pHSLdelta2 from pHSL
RC p70A1 from pHSLdelta1 & sAC-1delta
RC p70A2 from pHSLdelta2 & sAC-1delta
RC pC70A1 from p70A1 & Carnegie 20 or car20
RC pC70A2 from p70A2 & Carnegie 20 or car20
RA Klemenz R., Hultmark D., Gehring W.J.;
RT "Selective translation of heat shock mRNA in Drosophila
RT melanogaster depends on sequence information in the leader";
RL EMBO J. 4:2053-2060(1985).
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RN [5]
RC p111F2, p56H8, p132E3 from pSF2124 & Drosophila hsp70 gene
RA Schedl P., Artavanis-Tsakonas S., Steward R., Gehring W.,
RA Mirault M.E., Goldschmidt-Clermont M., Moran L., Tissieres A.;
RT "Two hybrid plasmids with D. melanogaster DNA sequences
RT complementary to mRNA coding for the major heat shock protein";
RL Cell 14:921-929(1978).
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RN [6]
RC pAG1 from pSKS106 & act88F gene
RC pAGH1 from pAG1 & polyA
RC pCSRH from pAT153 & P element
RC p75RH from pAT153 & KM75 gene
RC pHSS6 from pUC18 & HH5 gene
RC P[ry.75A], P[ry.75B], P[ry.75C] from Carnegie 20 & act88F gene
RC P[ry.75D], P[ry.75E], P[ry.75F] from Carnegie 20 & act88F gene
RC P[ry.CSA], P[ry.CSB], P[ry.CSC] from Carnegie 20 & act88F gene
RC P[ry.CS-75], P[ry.75-CS], P[ry.CS-5] from Carnegie 20 & act88F gene
RC P[ry.CSdeltaK], P[ry.AGH] from Carnegie 20 & act88F gene
RC P[ry.AGH] from Carnegie 20 & pAGH1
RA Hiromi Y., Okamoto H., Gehring W.J., Hotta Y.;
RT "Germline transformation with Drosophila mutant actin genes
RT induces constitutive expression of heat shock genes";
RL Cell 44:293-301(1986).
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RN [7]
RC pAT365.2 from pAT153 & pWP-2
RA Fjose A.;
RT ;
RL Unpublished (1984).
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RN [8]
RC sAC-1 from ADH2 gene
RA Goldberg D.A.;
RT "Isolation and partial characterization of the Drosophila alcohol
RT dehydrogenase gene";
RL Proc. Natl. Acad. Sci. U.S.A. 77:5794-5798(1980).
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CC P element mediates germ line transformation of Drosophila
CC melanogaster.
CC NCBI gi: 58137
CC NM (pW8)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (Drosophila melanogaster P element)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pSF2124 R1 10900bp
FT poly(dT) connector
FT 2. Drosophila hsp70 gene
FT poly(dAT) connector
FT -> p56H8
FT 1. pAT153 remove EcoRI-BamHI 377bp 3657..3658..376,
FT \ 3281bp
FT 2. p56H8 EcoRI-BamHI, hsp70 gene
FT -> pHSL
FT 1. p6.1 remove EcoRI-BamHI 377bp 4360..4361..376,
FT \ [375bp]
FT S1 nuclease
FT XhoI, Drosophila
FT Klenow
FT 2. pUC9 HindIII-HaeIII/FnuDI 40bp 234..274
FT Klenow
FT -> p6.3 [unique EcoRI, SamI, BamHI in P element]
FT 1. phage M558 EcoRI-EcoRI 12000bp, w+ locus
FT 2. p6.3 EcoRI, MCS
FT -> pWP-2
FT 1. pUC18 SalI 2686bp 418..418
FT Klenow:Klenow
FT 2. pWP-2 MaeI-HindIII 535bp 3707..3172
FT Klenow:Klenow
FT -> pW1 3220bp
FT 1. pW1 XbaI-EcoRI
FT 2. pWP-2 KpnI, 3' to white gene
FT BAL31 nuclease, remove 2200bp at 4400
FT XbaI-EcoRI
FT -> pW2
FT 1. pW2 XbaI
FT Klenow
FT -> pW3 [no XbaI]
FT 1. pW3 PstI
FT T4 DNA polymerase:T4 DNA polymerase
FT 2. pHSL HaeIII-XmnI 388bp, hsp70 promoter/leader
FT -> pW4
FT 1. Carnegie 4 SalI
FT fill in
FT XhoI linker 8bp cctcgagg
FT SacI
FT T4 DNA polymerase
FT KpnI linker 8bp gggtaccc
FT BamHI
FT fill in
FT BglII linker 8bp cagatctg
FT -> car4.3
FT 1. pW4 HindIII-EcoRI
FT Klenow:Klenow
FT 2. car4.3 HindIII, MCS
FT Klenow:Klenow
FT -> pW5
FT 1. pW5 XhoI
FT fill in
FT HpaI linker 6bp gttaac
FT -> pW7
FT 1. pW7 BglII
FT 2. NotI-SacII-SfiI-SpeI linker 147bp
FT \ gaattcggtacccgcccgggatcagatccgcggccgcataggccactagt
FT \ ggatctggatcctctagagtcgacctcgaacgttaacgttaacgtaacgt
FT \ taactcgaggcctcgaggtcgacctgcagccaagctacgttgcatgc
FT -> pW8"
FT misc_feature 1..6
FT /note="SIT unique EcoRI"
FT misc_feature 7..12
FT /note="SIT unique KpnI"
FT misc_feature 15..20
FT /note="SIT unique SmaI"
FT misc_feature 28..33
FT /note="SIT unique SacII"
FT misc_feature 30..37
FT /note="SIT unique NotI"
FT misc_feature 32..44
FT /note="SIT unique SfiI"
FT misc_feature 45..50
FT /note="SIT unique SpeI"
FT misc_feature 57..62
FT /note="SIT unique BamHI"
FT misc_feature 63..68
FT /note="SIT unique XbaI"
FT misc_feature 69..74
FT /note="SIT SalI"
FT misc_feature 82..87
FT /note="SIT HpaI"
FT misc_feature 88..93
FT /note="SIT HpaI"
FT misc_feature 99..104
FT /note="SIT HpaI"
FT misc_feature 104..109
FT /note="SIT XhoI"
FT misc_feature 108..113
FT /note="SIT unique StuI"
FT misc_feature 112..117
FT /note="SIT XhoI"
FT misc_feature 118..123
FT /note="SIT SalI"
FT misc_feature 124..129
FT /note="SIT unique PstI"
FT misc_feature 142..147
FT /note="SIT unique SphI"
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SQ Sequence 147 BP; 33 A; 44 C; 40 G; 30 T; 0 other;
gaattcggta cccgcccggg atcagatccg cggccgcata ggccactagt ggatctggat
cctctagagt cgacctcgaa cgttaacgtt aacgtaacgt taactcgagg cctcgaggtc
gacctgcagc caagctacgt tgcatgc
//