ID   VTK79L     preliminary; circular DNA; SYN; 1 BP.
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AC   ATCCVR2056;
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DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vaccinia virus vector VTK-79L - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   vP-7, vP-8 from pBR322 & vaccinia virus VTK-79
RC   vaccinia virus VTK-79 from vaccinia virus WR
RC   vaccinia virus VTK-79L from vaccinia virus WR & vaccinia virus VTK-79
RA   ;
RT   ;
RL   Unpublished (1986).
RL   U.S. Patent No. 4,603,112 dated July 29, 1986.
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RN   [2]
RC   vP-7, vP-8 from pBR322 & vaccinia virus VTK-79
RC   vaccinia virus VTK-79 from vaccinia virus WR
RC   vaccinia virus VTK-79L from vaccinia virus WR & vaccinia virus VTK-79
RA   ;
RT   ;
RL   Unpublished (1988).
RL   U.S. Patent No. 4,722,848 dated Feb. 2, 1988.
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CC   The HindIII J-fragment of TK-79 containing a mutant (non-functional)
CC   thymidine kinase (TK) gene was cloned into the L-variant vaccinia
CC   virus by in vivo recombination. [2]
CC   This is a thymidine kinase negative (TK-) mutant of the L-variant of
CC   vaccinia virus strain WR.  Plasmids can be introduced into this
CC   mutant by in vivo recombination. [2]
CC   NM (vaccinia virus VTK-79L)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (virus)
CC   SP (ATCC)
CC   HO (vaccinia virus)(BHK-21 cells)(chicken embryo fibroblasts)
CC   HO (monkey CV-1 cells)(HeLa cells)(Vero cells)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. vaccinia virus WR
FT                   2. vaccinia virus VTK-79
FT                   -> vaccinia virus VTK-79L"
FT   rep_origin      0..0
FT                   /note="ORI vaccinia virus"
FT   promoter        0..0
FT                   /note="PRO vaccinia genes"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//